Results 1 - 20 of 51 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23504 | 3' | -56 | NC_005259.1 | + | 17334 | 0.71 | 0.398954 |
Target: 5'- gGUGuGCCGGucaacgucgccgucGAgGUCGGCG-GCUCGGCg -3' miRNA: 3'- -UAC-UGGCC--------------UUgUAGCCGCuCGAGCUG- -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 60072 | 0.66 | 0.747367 |
Target: 5'- -aGGCuCGGGcgGC-UCgccgacuuucucgauGGCGAGCUCGACc -3' miRNA: 3'- uaCUG-GCCU--UGuAG---------------CCGCUCGAGCUG- -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 42550 | 0.69 | 0.539873 |
Target: 5'- gGUGGCgGGcAGC-UCGGCGAGCagccggucgucaUCGAUa -3' miRNA: 3'- -UACUGgCC-UUGuAGCCGCUCG------------AGCUG- -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 11569 | 0.69 | 0.539873 |
Target: 5'- uUGAUCGGuAGCAaaaUCGuaGCGGGCUUGGCc -3' miRNA: 3'- uACUGGCC-UUGU---AGC--CGCUCGAGCUG- -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 47886 | 0.69 | 0.539873 |
Target: 5'- uUGGCCGGGucaGCGUCGGCucguuGAGCgccUUGAUg -3' miRNA: 3'- uACUGGCCU---UGUAGCCG-----CUCG---AGCUG- -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 67835 | 0.7 | 0.498349 |
Target: 5'- -cGACCGGcAGCc-CGGUGAGCgggUCGACc -3' miRNA: 3'- uaCUGGCC-UUGuaGCCGCUCG---AGCUG- -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 54488 | 0.7 | 0.488187 |
Target: 5'- --cGCCGaGGGuCAUCGGCGGGCauggCGGCg -3' miRNA: 3'- uacUGGC-CUU-GUAGCCGCUCGa---GCUG- -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 7627 | 0.7 | 0.468164 |
Target: 5'- -gGGCCGGAccuacacCGUCGcCGAGUUCGACc -3' miRNA: 3'- uaCUGGCCUu------GUAGCcGCUCGAGCUG- -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 4130 | 0.71 | 0.438946 |
Target: 5'- cGUGGCCaaGAcCGUCGGCGAGUUCaagGACg -3' miRNA: 3'- -UACUGGc-CUuGUAGCCGCUCGAG---CUG- -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 9355 | 0.71 | 0.43799 |
Target: 5'- gAUGACCgucuguaGGucguAC-UCGGCGAGCUCGGa -3' miRNA: 3'- -UACUGG-------CCu---UGuAGCCGCUCGAGCUg -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 27385 | 0.71 | 0.42944 |
Target: 5'- gGUGACCGGcggcggugucGGCAgCGGCGgcAGCUCGGu -3' miRNA: 3'- -UACUGGCC----------UUGUaGCCGC--UCGAGCUg -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 10413 | 0.8 | 0.117182 |
Target: 5'- -aGGCCcuuGAGCAccguccUCGGCGAGCUCGACa -3' miRNA: 3'- uaCUGGc--CUUGU------AGCCGCUCGAGCUG- -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 46647 | 0.72 | 0.383799 |
Target: 5'- cAUGGCCacacGGcuGCGguugagguucUCGGCGAGCUCGGCc -3' miRNA: 3'- -UACUGG----CCu-UGU----------AGCCGCUCGAGCUG- -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 60813 | 0.73 | 0.310147 |
Target: 5'- -aGGCCGGAAauCGUCGGCGAuGCU-GGCc -3' miRNA: 3'- uaCUGGCCUU--GUAGCCGCU-CGAgCUG- -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 41783 | 0.74 | 0.302661 |
Target: 5'- --cACCGG--CAUCGGCGAGCagUCGGCa -3' miRNA: 3'- uacUGGCCuuGUAGCCGCUCG--AGCUG- -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 8377 | 0.74 | 0.302661 |
Target: 5'- -cGGCCucGGCAUCGGCGAGagcCUCGGCg -3' miRNA: 3'- uaCUGGccUUGUAGCCGCUC---GAGCUG- -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 62771 | 0.74 | 0.295315 |
Target: 5'- -gGGCCGGAACGUCGcGC-AGCUUGGu -3' miRNA: 3'- uaCUGGCCUUGUAGC-CGcUCGAGCUg -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 66942 | 0.74 | 0.295315 |
Target: 5'- gGUGAgCGGGACAcCGGCGAGCUg--- -3' miRNA: 3'- -UACUgGCCUUGUaGCCGCUCGAgcug -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 29400 | 0.74 | 0.281041 |
Target: 5'- --cACCGaGGGCcUCGGCGAGCUCGuGCg -3' miRNA: 3'- uacUGGC-CUUGuAGCCGCUCGAGC-UG- -5' |
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23504 | 3' | -56 | NC_005259.1 | + | 5632 | 0.75 | 0.24776 |
Target: 5'- -cGACCGGAuCGUgccCGGCGAGgaCGGCg -3' miRNA: 3'- uaCUGGCCUuGUA---GCCGCUCgaGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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