Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23506 | 5' | -52.8 | NC_005259.1 | + | 24407 | 0.66 | 0.894342 |
Target: 5'- aCCGuGcucaaUGACCucggcgagGAAGCGACagCGGGCa -3' miRNA: 3'- aGGC-C-----ACUGGua------CUUCGUUGa-GUCCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 20977 | 0.66 | 0.894342 |
Target: 5'- gCCGGggUGACaacugCGUGuGGGCcuACUCGGGCg -3' miRNA: 3'- aGGCC--ACUG-----GUAC-UUCGu-UGAGUCCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 45493 | 0.66 | 0.887076 |
Target: 5'- gCUGGUGGCCuu--GGUcGCUguGGCc -3' miRNA: 3'- aGGCCACUGGuacuUCGuUGAguCCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 46623 | 0.66 | 0.887076 |
Target: 5'- cUUGGUGGCC-UGAcuGGuCAGCUCcauGGCc -3' miRNA: 3'- aGGCCACUGGuACU--UC-GUUGAGu--CCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 35189 | 0.66 | 0.886335 |
Target: 5'- gCCGGUGAgCAgcaccgugccgccUGAGGCGGCg-AGGa -3' miRNA: 3'- aGGCCACUgGU-------------ACUUCGUUGagUCCg -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 62698 | 0.66 | 0.879548 |
Target: 5'- -gCGGUGucCCAUccGGCGA-UCAGGCg -3' miRNA: 3'- agGCCACu-GGUAcuUCGUUgAGUCCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 45523 | 0.66 | 0.879548 |
Target: 5'- gCCGGUGuuggccGCCGUGGcugcgAGCGgguuGCUCgccGGGCc -3' miRNA: 3'- aGGCCAC------UGGUACU-----UCGU----UGAG---UCCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 54008 | 0.66 | 0.879548 |
Target: 5'- gCCGGgGuCCAcc-AGCGacGCUCGGGCg -3' miRNA: 3'- aGGCCaCuGGUacuUCGU--UGAGUCCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 52400 | 0.66 | 0.871764 |
Target: 5'- cUCGG--GCCAUcGAGGUgugggcgcucGACUCGGGCg -3' miRNA: 3'- aGGCCacUGGUA-CUUCG----------UUGAGUCCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 58953 | 0.66 | 0.871764 |
Target: 5'- gUCGGccuUGGCCAgcagGucgGGCAGCUCGucGGCg -3' miRNA: 3'- aGGCC---ACUGGUa---Cu--UCGUUGAGU--CCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 51839 | 0.66 | 0.871764 |
Target: 5'- cCCGGUugaucGGCCAcucGaAAGUcacgAGCUCAGGCu -3' miRNA: 3'- aGGCCA-----CUGGUa--C-UUCG----UUGAGUCCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 60507 | 0.66 | 0.869379 |
Target: 5'- gCCGGUGuugucguagugcGCCAUGGccgggccgggacgcAGCcGCUCGucGGCa -3' miRNA: 3'- aGGCCAC------------UGGUACU--------------UCGuUGAGU--CCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 57677 | 0.66 | 0.863728 |
Target: 5'- gUCCGGccaaucGACCGUGGccaGGUuGC-CGGGCu -3' miRNA: 3'- -AGGCCa-----CUGGUACU---UCGuUGaGUCCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 44804 | 0.66 | 0.863728 |
Target: 5'- gCCGGUGGCCuggcccuUGGucAGCAGCUCc--- -3' miRNA: 3'- aGGCCACUGGu------ACU--UCGUUGAGuccg -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 59426 | 0.67 | 0.85545 |
Target: 5'- gUCGGcGGCCAUGAagaacGGCggUUCguuacgGGGCg -3' miRNA: 3'- aGGCCaCUGGUACU-----UCGuuGAG------UCCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 61028 | 0.67 | 0.846936 |
Target: 5'- cUCGGUGGCgGcGAGGuCGACcgaCGGGCg -3' miRNA: 3'- aGGCCACUGgUaCUUC-GUUGa--GUCCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 58664 | 0.67 | 0.846936 |
Target: 5'- cCCGGUGcACCggGguGCAGCUUccaucgcgGGGUc -3' miRNA: 3'- aGGCCAC-UGGuaCuuCGUUGAG--------UCCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 23260 | 0.67 | 0.838195 |
Target: 5'- -gCGGUGGCCAccaUGA-GCAccaagAC-CGGGCg -3' miRNA: 3'- agGCCACUGGU---ACUuCGU-----UGaGUCCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 34873 | 0.69 | 0.750908 |
Target: 5'- gUCGGUG-CCGgaccGggGUGcCUCGGGCa -3' miRNA: 3'- aGGCCACuGGUa---CuuCGUuGAGUCCG- -5' |
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23506 | 5' | -52.8 | NC_005259.1 | + | 10251 | 0.69 | 0.74045 |
Target: 5'- -gCGGUGugCuUGAugAGCGGgccgguCUCAGGCa -3' miRNA: 3'- agGCCACugGuACU--UCGUU------GAGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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