Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23508 | 5' | -64.5 | NC_005259.1 | + | 60053 | 0.66 | 0.32522 |
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Target: 5'- -uCGGGGUcuucucgggcucaGGCUcgggCGGCUCGCCGAcuuucucgauGGCg -3' miRNA: 3'- ucGCUCCA-------------CCGG----GCCGAGCGGCU----------CCG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 61603 | 0.68 | 0.245762 |
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Target: 5'- cGGUGGGGUcGGCggggaacagcagaCCGGCacCGCCGAcGGUg -3' miRNA: 3'- -UCGCUCCA-CCG-------------GGCCGa-GCGGCU-CCG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 63658 | 0.66 | 0.326694 |
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Target: 5'- uGGCcGGGacgccggacaccucgGGCaCCGGCgCGCCGGGGa -3' miRNA: 3'- -UCGcUCCa--------------CCG-GGCCGaGCGGCUCCg -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 63860 | 0.79 | 0.036688 |
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Target: 5'- cGGCGAGGUGG-CCGGCUgGCCuugcguGGGCg -3' miRNA: 3'- -UCGCUCCACCgGGCCGAgCGGc-----UCCG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 64766 | 0.67 | 0.290648 |
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Target: 5'- cGCGAcGGUGcGCCuCGGCggccCGCUGcgacuGGCu -3' miRNA: 3'- uCGCU-CCAC-CGG-GCCGa---GCGGCu----CCG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 64943 | 0.69 | 0.202738 |
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Target: 5'- cGCGAgGGUGGCCgGGUU-GUCGAuGGUc -3' miRNA: 3'- uCGCU-CCACCGGgCCGAgCGGCU-CCG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 65430 | 0.7 | 0.171955 |
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Target: 5'- aAGCGA--UGGCCUGaGCUCGCaccugcgaacaacaaCGAGGCc -3' miRNA: 3'- -UCGCUccACCGGGC-CGAGCG---------------GCUCCG- -5' |
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| 23508 | 5' | -64.5 | NC_005259.1 | + | 68844 | 0.66 | 0.345539 |
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Target: 5'- cGGUGAGGUGGgCCaucaugucgacuuGCccgCGCCGGGcGCg -3' miRNA: 3'- -UCGCUCCACCgGGc------------CGa--GCGGCUC-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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