Results 21 - 40 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23508 | 5' | -64.5 | NC_005259.1 | + | 32490 | 0.68 | 0.264613 |
Target: 5'- cGCGAGGUGGUucuguaugaacaCCGuGagguaGUCGAGGCg -3' miRNA: 3'- uCGCUCCACCG------------GGC-Cgag--CGGCUCCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 32900 | 0.7 | 0.183551 |
Target: 5'- gGGCGAgGGUGagccaCCCGGCcCGCUcGGGCg -3' miRNA: 3'- -UCGCU-CCACc----GGGCCGaGCGGcUCCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 33362 | 0.67 | 0.278038 |
Target: 5'- gGGCGAGGUcaugacucuccuaCCGGUcCGCCGAGcGCg -3' miRNA: 3'- -UCGCUCCAccg----------GGCCGaGCGGCUC-CG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 35656 | 0.66 | 0.341708 |
Target: 5'- uGGCGAgGGUGGCCagcgCGGUgguggugcacagcgaCGCCGugcccGGCg -3' miRNA: 3'- -UCGCU-CCACCGG----GCCGa--------------GCGGCu----CCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 36412 | 0.66 | 0.348625 |
Target: 5'- gAGCGccucaaGGGUcauGGUCUGcGCagcgUCGCCGAGGUc -3' miRNA: 3'- -UCGC------UCCA---CCGGGC-CG----AGCGGCUCCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 37760 | 0.69 | 0.212971 |
Target: 5'- cAGCGcGGUGGCCCcuGCggGCaCGAGcGCa -3' miRNA: 3'- -UCGCuCCACCGGGc-CGagCG-GCUC-CG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 42260 | 0.66 | 0.311462 |
Target: 5'- uGUGAGGUGGUCa----CGUCGAGGCc -3' miRNA: 3'- uCGCUCCACCGGgccgaGCGGCUCCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 42917 | 0.66 | 0.340946 |
Target: 5'- cGUu-GGUGGCCUugagcaugaGGCccUCGCCGuGGGCg -3' miRNA: 3'- uCGcuCCACCGGG---------CCG--AGCGGC-UCCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 45488 | 0.67 | 0.3044 |
Target: 5'- uGGCcgcuGGUGGCCUuGgUCGCUGuGGCc -3' miRNA: 3'- -UCGcu--CCACCGGGcCgAGCGGCuCCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 46676 | 0.66 | 0.318648 |
Target: 5'- cGGCGAGcucGGCCUGaGC-CGCCGccGCg -3' miRNA: 3'- -UCGCUCca-CCGGGC-CGaGCGGCucCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 47057 | 0.66 | 0.347852 |
Target: 5'- gAGCGuguuGGUGaGCgaGGCgagCGCCGAgacgaugGGCa -3' miRNA: 3'- -UCGCu---CCAC-CGggCCGa--GCGGCU-------CCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 47887 | 0.67 | 0.29268 |
Target: 5'- uGGCcGGGUcagcgucGGCUCguugagcgccuugauGGCgUCGCCGAGGCc -3' miRNA: 3'- -UCGcUCCA-------CCGGG---------------CCG-AGCGGCUCCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 48051 | 0.66 | 0.340946 |
Target: 5'- ----cGGUGGCCaCGGCggguagCGCCGccGCg -3' miRNA: 3'- ucgcuCCACCGG-GCCGa-----GCGGCucCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 50374 | 0.76 | 0.063003 |
Target: 5'- cGGCGAGaaacucGGCCCacgugucgcGGCUCGCCGAGcGCg -3' miRNA: 3'- -UCGCUCca----CCGGG---------CCGAGCGGCUC-CG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 53588 | 0.68 | 0.229148 |
Target: 5'- cGUGAGGUGuuCCuuuuuGCUCGUCGGGGUc -3' miRNA: 3'- uCGCUCCACcgGGc----CGAGCGGCUCCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 54704 | 0.71 | 0.142463 |
Target: 5'- aGGCG-GGUGGCCUcagccugguacaGGacauaGCCGAGGCu -3' miRNA: 3'- -UCGCuCCACCGGG------------CCgag--CGGCUCCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 56405 | 0.66 | 0.311462 |
Target: 5'- -uCGAuGGU-GCCCGGCcCGCCGAGcucaaGCa -3' miRNA: 3'- ucGCU-CCAcCGGGCCGaGCGGCUC-----CG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 56701 | 0.71 | 0.142463 |
Target: 5'- uGCGAGGUGGCCacgauGGUgUCcCCGAGcGCg -3' miRNA: 3'- uCGCUCCACCGGg----CCG-AGcGGCUC-CG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 57445 | 0.67 | 0.270939 |
Target: 5'- cGGCGAGGuUGGUgcgacgcugCGGCagGCCGGuGGCa -3' miRNA: 3'- -UCGCUCC-ACCGg--------GCCGagCGGCU-CCG- -5' |
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23508 | 5' | -64.5 | NC_005259.1 | + | 58691 | 0.69 | 0.201241 |
Target: 5'- cGCGGGGUcGGCUCGucGCcgccgccgucaccaUCGCCGGGGg -3' miRNA: 3'- uCGCUCCA-CCGGGC--CG--------------AGCGGCUCCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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