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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23509 | 5' | -52.2 | NC_005259.1 | + | 2363 | 0.71 | 0.680992 |
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Target: 5'- gGGCGGGGUUUUCuguaucacaggcagGGUUgacauacccgacaUAGGUGGGu -3' miRNA: 3'- gCCGCCCCAAAAG--------------CCAA-------------AUCCGCUUc -5' |
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| 23509 | 5' | -52.2 | NC_005259.1 | + | 7039 | 0.72 | 0.608493 |
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Target: 5'- cCGGCGGGGUUUUUcGUUgc-GCGAGa -3' miRNA: 3'- -GCCGCCCCAAAAGcCAAaucCGCUUc -5' |
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| 23509 | 5' | -52.2 | NC_005259.1 | + | 7793 | 0.69 | 0.783888 |
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Target: 5'- cCGGCGGGGUUUUcucguuuguaaugacCGGUaaugacccGGCGgcGg -3' miRNA: 3'- -GCCGCCCCAAAA---------------GCCAaau-----CCGCuuC- -5' |
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| 23509 | 5' | -52.2 | NC_005259.1 | + | 13314 | 0.67 | 0.88557 |
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Target: 5'- gCGGCGGGGUauaucUUGGUaacccacGGCGGGa -3' miRNA: 3'- -GCCGCCCCAaa---AGCCAaau----CCGCUUc -5' |
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| 23509 | 5' | -52.2 | NC_005259.1 | + | 67216 | 0.66 | 0.921166 |
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Target: 5'- uCGGCGGGGUcgaugccgaccacggCGGUgUAGGgGuuGa -3' miRNA: 3'- -GCCGCCCCAaaa------------GCCAaAUCCgCuuC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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