miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23509 5' -52.2 NC_005259.1 + 67216 0.66 0.921166
Target:  5'- uCGGCGGGGUcgaugccgaccacggCGGUgUAGGgGuuGa -3'
miRNA:   3'- -GCCGCCCCAaaa------------GCCAaAUCCgCuuC- -5'
23509 5' -52.2 NC_005259.1 + 13314 0.67 0.88557
Target:  5'- gCGGCGGGGUauaucUUGGUaacccacGGCGGGa -3'
miRNA:   3'- -GCCGCCCCAaa---AGCCAaau----CCGCUUc -5'
23509 5' -52.2 NC_005259.1 + 7793 0.69 0.783888
Target:  5'- cCGGCGGGGUUUUcucguuuguaaugacCGGUaaugacccGGCGgcGg -3'
miRNA:   3'- -GCCGCCCCAAAA---------------GCCAaau-----CCGCuuC- -5'
23509 5' -52.2 NC_005259.1 + 7039 0.72 0.608493
Target:  5'- cCGGCGGGGUUUUUcGUUgc-GCGAGa -3'
miRNA:   3'- -GCCGCCCCAAAAGcCAAaucCGCUUc -5'
23509 5' -52.2 NC_005259.1 + 2363 0.71 0.680992
Target:  5'- gGGCGGGGUUUUCuguaucacaggcagGGUUgacauacccgacaUAGGUGGGu -3'
miRNA:   3'- gCCGCCCCAAAAG--------------CCAA-------------AUCCGCUUc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.