Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23510 | 3' | -58.5 | NC_005259.1 | + | 2082 | 0.69 | 0.451327 |
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Target: 5'- gGGCGGgccUGCuCGAuuuCAUCGGCGAGGccaaUUGGc -3' miRNA: 3'- -CUGCC---ACG-GCU---GUAGCCGCUCC----AGCC- -5' |
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| 23510 | 3' | -58.5 | NC_005259.1 | + | 41774 | 0.69 | 0.451327 |
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Target: 5'- cGCGaacaacaCCGGCAUCGGCGAGcaGUCGGc -3' miRNA: 3'- cUGCcac----GGCUGUAGCCGCUC--CAGCC- -5' |
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| 23510 | 3' | -58.5 | NC_005259.1 | + | 41638 | 0.69 | 0.470247 |
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Target: 5'- uGGCGGUGCCGgucugcggGCGcUCGGUGAG--CGGg -3' miRNA: 3'- -CUGCCACGGC--------UGU-AGCCGCUCcaGCC- -5' |
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| 23510 | 3' | -58.5 | NC_005259.1 | + | 68802 | 0.69 | 0.476001 |
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Target: 5'- gGGCGGUGUCGgugcccgguacauGCucucggucaaagcaGUCGGUGAGGUgGGc -3' miRNA: 3'- -CUGCCACGGC-------------UG--------------UAGCCGCUCCAgCC- -5' |
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| 23510 | 3' | -58.5 | NC_005259.1 | + | 32637 | 0.69 | 0.489562 |
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Target: 5'- -uCGGUacGCCG-CAUUGGcCGGGGUgGGg -3' miRNA: 3'- cuGCCA--CGGCuGUAGCC-GCUCCAgCC- -5' |
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| 23510 | 3' | -58.5 | NC_005259.1 | + | 66664 | 0.68 | 0.498375 |
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Target: 5'- cGGCGGUGCUGcgaugcuCAUCGuGCcgcccauGAGGUCGa -3' miRNA: 3'- -CUGCCACGGCu------GUAGC-CG-------CUCCAGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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