Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23512 | 5' | -57.7 | NC_005259.1 | + | 1677 | 0.68 | 0.513643 |
Target: 5'- --aGGUCCGaucUGCGC-GCCCGCaagCGCGa -3' miRNA: 3'- gaaCCGGGU---AUGCGaCGGGUGa--GCGU- -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 7689 | 0.76 | 0.157373 |
Target: 5'- -gUGGCCCGUACGuCUGCaCCACcaUCGUc -3' miRNA: 3'- gaACCGGGUAUGC-GACG-GGUG--AGCGu -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 9777 | 0.67 | 0.565987 |
Target: 5'- -cUGGCUCGacuucUGCccggugUGCCCGCUCGCGc -3' miRNA: 3'- gaACCGGGUau---GCG------ACGGGUGAGCGU- -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 10133 | 0.68 | 0.483196 |
Target: 5'- ---aGCCCAcGCGCgcGCCCGCcguUCGCAc -3' miRNA: 3'- gaacCGGGUaUGCGa-CGGGUG---AGCGU- -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 20519 | 0.66 | 0.641181 |
Target: 5'- -gUGuCCCgagcacggGUGCGCUGCCCGCagCGUg -3' miRNA: 3'- gaACcGGG--------UAUGCGACGGGUGa-GCGu -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 22847 | 0.67 | 0.534371 |
Target: 5'- --cGGCCCAccCGCaGCCgcaaccugUACUCGCAg -3' miRNA: 3'- gaaCCGGGUauGCGaCGG--------GUGAGCGU- -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 24332 | 0.69 | 0.453644 |
Target: 5'- --cGGCCuCAcGCGCUgGCCUaccgccggguGCUCGCAc -3' miRNA: 3'- gaaCCGG-GUaUGCGA-CGGG----------UGAGCGU- -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 24849 | 0.68 | 0.503402 |
Target: 5'- ---aGUCCGUGCGCUacgccgagGCCCGcCUCGCu -3' miRNA: 3'- gaacCGGGUAUGCGA--------CGGGU-GAGCGu -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 27732 | 0.67 | 0.555387 |
Target: 5'- aCUUGuucgcGCCCGc-UGCUGCCCACggUGCAu -3' miRNA: 3'- -GAAC-----CGGGUauGCGACGGGUGa-GCGU- -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 29936 | 0.7 | 0.380005 |
Target: 5'- -gUGGCCgccACGCgGCCCGgUCGCGg -3' miRNA: 3'- gaACCGGguaUGCGaCGGGUgAGCGU- -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 30573 | 0.67 | 0.52812 |
Target: 5'- cCUUGGCCgCAguaGCGCUguuguccucccacacGCCCACggucUCGCc -3' miRNA: 3'- -GAACCGG-GUa--UGCGA---------------CGGGUG----AGCGu -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 32300 | 0.68 | 0.511588 |
Target: 5'- cCUUGGCCCAgagccuugagcCGCagUGCCCGCcaauugugCGCGg -3' miRNA: 3'- -GAACCGGGUau---------GCG--ACGGGUGa-------GCGU- -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 35085 | 0.66 | 0.63039 |
Target: 5'- -gUGGCCU--GCGCaGUCCACaaGCAg -3' miRNA: 3'- gaACCGGGuaUGCGaCGGGUGagCGU- -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 37456 | 0.73 | 0.239507 |
Target: 5'- aUUGGCCCgAUugGCUGgCCACgcCGCc -3' miRNA: 3'- gAACCGGG-UAugCGACgGGUGa-GCGu -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 41386 | 0.67 | 0.543796 |
Target: 5'- gCUUGGCCgGUacccaugGCGCgccgcucggggUGCCCACgaugUGCAc -3' miRNA: 3'- -GAACCGGgUA-------UGCG-----------ACGGGUGa---GCGU- -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 44236 | 0.67 | 0.565987 |
Target: 5'- ---cGCCCGccGCGCcGCCCGCgCGCGa -3' miRNA: 3'- gaacCGGGUa-UGCGaCGGGUGaGCGU- -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 49658 | 0.68 | 0.473239 |
Target: 5'- --cGGCCUcgAUACGC-GCCUGCUCGg- -3' miRNA: 3'- gaaCCGGG--UAUGCGaCGGGUGAGCgu -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 50510 | 0.67 | 0.522932 |
Target: 5'- -gUGGgCgGUGCGCUGCaCCucgacgcGCUCGCc -3' miRNA: 3'- gaACCgGgUAUGCGACG-GG-------UGAGCGu -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 54393 | 0.67 | 0.523968 |
Target: 5'- gCUUGGCCUGUuccuCGCgGauuuugucgauaUCCGCUCGCAg -3' miRNA: 3'- -GAACCGGGUAu---GCGaC------------GGGUGAGCGU- -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 57693 | 0.67 | 0.576638 |
Target: 5'- -gUGGCCagguUGCcggGCuUGCCCgACUCGCGg -3' miRNA: 3'- gaACCGGgu--AUG---CG-ACGGG-UGAGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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