Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 23512 | 5' | -57.7 | NC_005259.1 | + | 22847 | 0.67 | 0.534371 |
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Target: 5'- --cGGCCCAccCGCaGCCgcaaccugUACUCGCAg -3' miRNA: 3'- gaaCCGGGUauGCGaCGG--------GUGAGCGU- -5' |
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| 23512 | 5' | -57.7 | NC_005259.1 | + | 30573 | 0.67 | 0.52812 |
|
Target: 5'- cCUUGGCCgCAguaGCGCUguuguccucccacacGCCCACggucUCGCc -3' miRNA: 3'- -GAACCGG-GUa--UGCGA---------------CGGGUG----AGCGu -5' |
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| 23512 | 5' | -57.7 | NC_005259.1 | + | 54393 | 0.67 | 0.523968 |
|
Target: 5'- gCUUGGCCUGUuccuCGCgGauuuugucgauaUCCGCUCGCAg -3' miRNA: 3'- -GAACCGGGUAu---GCGaC------------GGGUGAGCGU- -5' |
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| 23512 | 5' | -57.7 | NC_005259.1 | + | 65629 | 0.66 | 0.587333 |
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Target: 5'- -aUGGCCCAUGaucgGCUGCaCCuuuCUCaGCc -3' miRNA: 3'- gaACCGGGUAUg---CGACG-GGu--GAG-CGu -5' |
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| 23512 | 5' | -57.7 | NC_005259.1 | + | 35085 | 0.66 | 0.63039 |
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Target: 5'- -gUGGCCU--GCGCaGUCCACaaGCAg -3' miRNA: 3'- gaACCGGGuaUGCGaCGGGUGagCGU- -5' |
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| 23512 | 5' | -57.7 | NC_005259.1 | + | 20519 | 0.66 | 0.641181 |
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Target: 5'- -gUGuCCCgagcacggGUGCGCUGCCCGCagCGUg -3' miRNA: 3'- gaACcGGG--------UAUGCGACGGGUGa-GCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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