Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23512 | 5' | -57.7 | NC_005259.1 | + | 44236 | 0.67 | 0.565987 |
Target: 5'- ---cGCCCGccGCGCcGCCCGCgCGCGa -3' miRNA: 3'- gaacCGGGUa-UGCGaCGGGUGaGCGU- -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 9777 | 0.67 | 0.565987 |
Target: 5'- -cUGGCUCGacuucUGCccggugUGCCCGCUCGCGc -3' miRNA: 3'- gaACCGGGUau---GCG------ACGGGUGAGCGU- -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 57693 | 0.67 | 0.576638 |
Target: 5'- -gUGGCCagguUGCcggGCuUGCCCgACUCGCGg -3' miRNA: 3'- gaACCGGgu--AUG---CG-ACGGG-UGAGCGU- -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 65629 | 0.66 | 0.587333 |
Target: 5'- -aUGGCCCAUGaucgGCUGCaCCuuuCUCaGCc -3' miRNA: 3'- gaACCGGGUAUg---CGACG-GGu--GAG-CGu -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 35085 | 0.66 | 0.63039 |
Target: 5'- -gUGGCCU--GCGCaGUCCACaaGCAg -3' miRNA: 3'- gaACCGGGuaUGCGaCGGGUGagCGU- -5' |
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23512 | 5' | -57.7 | NC_005259.1 | + | 20519 | 0.66 | 0.641181 |
Target: 5'- -gUGuCCCgagcacggGUGCGCUGCCCGCagCGUg -3' miRNA: 3'- gaACcGGG--------UAUGCGACGGGUGa-GCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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