Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23512 | 5' | -57.7 | NC_005259.1 | + | 29936 | 0.7 | 0.380005 |
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Target: 5'- -gUGGCCgccACGCgGCCCGgUCGCGg -3' miRNA: 3'- gaACCGGguaUGCGaCGGGUgAGCGU- -5' |
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| 23512 | 5' | -57.7 | NC_005259.1 | + | 60733 | 0.71 | 0.314805 |
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Target: 5'- --cGGCCCGUGuCGggGUCCAUUUGCAg -3' miRNA: 3'- gaaCCGGGUAU-GCgaCGGGUGAGCGU- -5' |
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| 23512 | 5' | -57.7 | NC_005259.1 | + | 37456 | 0.73 | 0.239507 |
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Target: 5'- aUUGGCCCgAUugGCUGgCCACgcCGCc -3' miRNA: 3'- gAACCGGG-UAugCGACgGGUGa-GCGu -5' |
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| 23512 | 5' | -57.7 | NC_005259.1 | + | 62005 | 0.74 | 0.221763 |
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Target: 5'- --gGGCaCCGUGCGgUGuaCCCGCUCGCGc -3' miRNA: 3'- gaaCCG-GGUAUGCgAC--GGGUGAGCGU- -5' |
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| 23512 | 5' | -57.7 | NC_005259.1 | + | 7689 | 0.76 | 0.157373 |
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Target: 5'- -gUGGCCCGUACGuCUGCaCCACcaUCGUc -3' miRNA: 3'- gaACCGGGUAUGC-GACG-GGUG--AGCGu -5' |
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| 23512 | 5' | -57.7 | NC_005259.1 | + | 67094 | 0.78 | 0.116627 |
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Target: 5'- cCUUGGCCCAUugGUccgagguguacUGCCCGCU-GCGg -3' miRNA: 3'- -GAACCGGGUAugCG-----------ACGGGUGAgCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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