Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23512 | 5' | -57.7 | NC_005259.1 | + | 61187 | 0.69 | 0.453644 |
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Target: 5'- -aUGGCCCu--CGCcgGUCUGCUUGCAc -3' miRNA: 3'- gaACCGGGuauGCGa-CGGGUGAGCGU- -5' |
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| 23512 | 5' | -57.7 | NC_005259.1 | + | 49658 | 0.68 | 0.473239 |
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Target: 5'- --cGGCCUcgAUACGC-GCCUGCUCGg- -3' miRNA: 3'- gaaCCGGG--UAUGCGaCGGGUGAGCgu -5' |
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| 23512 | 5' | -57.7 | NC_005259.1 | + | 10133 | 0.68 | 0.483196 |
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Target: 5'- ---aGCCCAcGCGCgcGCCCGCcguUCGCAc -3' miRNA: 3'- gaacCGGGUaUGCGa-CGGGUG---AGCGU- -5' |
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| 23512 | 5' | -57.7 | NC_005259.1 | + | 24849 | 0.68 | 0.503402 |
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Target: 5'- ---aGUCCGUGCGCUacgccgagGCCCGcCUCGCu -3' miRNA: 3'- gaacCGGGUAUGCGA--------CGGGU-GAGCGu -5' |
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| 23512 | 5' | -57.7 | NC_005259.1 | + | 32300 | 0.68 | 0.511588 |
|
Target: 5'- cCUUGGCCCAgagccuugagcCGCagUGCCCGCcaauugugCGCGg -3' miRNA: 3'- -GAACCGGGUau---------GCG--ACGGGUGa-------GCGU- -5' |
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| 23512 | 5' | -57.7 | NC_005259.1 | + | 20519 | 0.66 | 0.641181 |
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Target: 5'- -gUGuCCCgagcacggGUGCGCUGCCCGCagCGUg -3' miRNA: 3'- gaACcGGG--------UAUGCGACGGGUGa-GCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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