Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23513 | 5' | -58.5 | NC_005259.1 | + | 36145 | 0.66 | 0.660704 |
Target: 5'- gCUGUCCGuCGC-CAUgGCCacgGUGACGAc -3' miRNA: 3'- -GGCAGGCcGCGaGUAgUGG---CGCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 20059 | 0.66 | 0.650304 |
Target: 5'- aUGUCCgcugcgguGGCGgUCGUCACCaCGuCGAa -3' miRNA: 3'- gGCAGG--------CCGCgAGUAGUGGcGCuGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 40761 | 0.66 | 0.650304 |
Target: 5'- aCGUCUGGUcgagcaucuGCUCGUCG--GCGAUGAc -3' miRNA: 3'- gGCAGGCCG---------CGAGUAGUggCGCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 60595 | 0.66 | 0.650304 |
Target: 5'- -aGUCgGGUGC-CGUCAUCgagcagcacagGCGGCGAg -3' miRNA: 3'- ggCAGgCCGCGaGUAGUGG-----------CGCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 27065 | 0.66 | 0.650304 |
Target: 5'- aCgGUCaCGGCGa-CAUCGCCgacauucuGCGGCGGc -3' miRNA: 3'- -GgCAG-GCCGCgaGUAGUGG--------CGCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 22697 | 0.66 | 0.650304 |
Target: 5'- cCCG-CCGuCGC-CGUCACCGgcuCGAUGAg -3' miRNA: 3'- -GGCaGGCcGCGaGUAGUGGC---GCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 42502 | 0.66 | 0.639889 |
Target: 5'- cCCGUCCGGCGgUguggccagcagcCGUCcgGCCcCGAUGGg -3' miRNA: 3'- -GGCAGGCCGCgA------------GUAG--UGGcGCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 14039 | 0.66 | 0.639889 |
Target: 5'- aUCGagCGGCaGCgCAUCGuCgGCGACGAg -3' miRNA: 3'- -GGCagGCCG-CGaGUAGU-GgCGCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 13045 | 0.66 | 0.639889 |
Target: 5'- aCGUUCGGCcCgu-UCugCGCGAUGGu -3' miRNA: 3'- gGCAGGCCGcGaguAGugGCGCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 17022 | 0.66 | 0.629468 |
Target: 5'- aCCGccaUCCucGGCGCUggCAUCgacaACCGCGACc- -3' miRNA: 3'- -GGC---AGG--CCGCGA--GUAG----UGGCGCUGcu -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 42446 | 0.67 | 0.598248 |
Target: 5'- gUGUUCGGCGUccaacaGUCGCCGacuaCGGCGAg -3' miRNA: 3'- gGCAGGCCGCGag----UAGUGGC----GCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 41645 | 0.67 | 0.571372 |
Target: 5'- gCCGgucugCgGGCGCUCGgugagcgggaucugCACCGCGuGCGu -3' miRNA: 3'- -GGCa----GgCCGCGAGUa-------------GUGGCGC-UGCu -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 14723 | 0.67 | 0.56726 |
Target: 5'- aCG-CC-GCGCUCGUCGCCGaGAUGu -3' miRNA: 3'- gGCaGGcCGCGAGUAGUGGCgCUGCu -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 23309 | 0.67 | 0.56726 |
Target: 5'- -gGUCCGGCGCggucggUGUCGCCGaGcCGGa -3' miRNA: 3'- ggCAGGCCGCGa-----GUAGUGGCgCuGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 49609 | 0.67 | 0.56726 |
Target: 5'- aCC-UCgGGCaGCUCAUCAUCGUcaucGGCGGc -3' miRNA: 3'- -GGcAGgCCG-CGAGUAGUGGCG----CUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 56566 | 0.68 | 0.557016 |
Target: 5'- -gGUUCGGUGaUCGUCgcgGCUGCGAUGAg -3' miRNA: 3'- ggCAGGCCGCgAGUAG---UGGCGCUGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 45518 | 0.68 | 0.546826 |
Target: 5'- aCCGcgCCGGUGUUgGcCGCCGUGGCu- -3' miRNA: 3'- -GGCa-GGCCGCGAgUaGUGGCGCUGcu -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 9503 | 0.68 | 0.536696 |
Target: 5'- -aGgaaCGuGCGCUCGUCGCgCGCGuCGAc -3' miRNA: 3'- ggCag-GC-CGCGAGUAGUG-GCGCuGCU- -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 17352 | 0.68 | 0.534677 |
Target: 5'- gCCGUCgaggucggcggcuCGGCGCUgGUCguggucgACUGCGACa- -3' miRNA: 3'- -GGCAG-------------GCCGCGAgUAG-------UGGCGCUGcu -5' |
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23513 | 5' | -58.5 | NC_005259.1 | + | 24053 | 0.68 | 0.526631 |
Target: 5'- gUCGUCggcgCGGUGCUCAaucucgugCACCGCGA-GAu -3' miRNA: 3'- -GGCAG----GCCGCGAGUa-------GUGGCGCUgCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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