miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23554 5' -52.2 NC_005260.1 + 44939 0.66 0.994668
Target:  5'- aGUCCACGGCGaauAUGAUcGCgauugaucuuaCAUUCGa -3'
miRNA:   3'- -UAGGUGCCGUgu-UACUAaCG-----------GUGAGC- -5'
23554 5' -52.2 NC_005260.1 + 188657 0.66 0.994589
Target:  5'- -gCCGCGGCAauugGAUaauuucuUGCUACUUGa -3'
miRNA:   3'- uaGGUGCCGUguuaCUA-------ACGGUGAGC- -5'
23554 5' -52.2 NC_005260.1 + 228457 0.67 0.982827
Target:  5'- aGUUCGCGGagcugcugaaAUAAUGcugcUUGCCACUCGa -3'
miRNA:   3'- -UAGGUGCCg---------UGUUACu---AACGGUGAGC- -5'
23554 5' -52.2 NC_005260.1 + 20373 0.68 0.978555
Target:  5'- uGUCCgacuuGCGGCACA--GAUUGCucuaauCACUCGc -3'
miRNA:   3'- -UAGG-----UGCCGUGUuaCUAACG------GUGAGC- -5'
23554 5' -52.2 NC_005260.1 + 217975 0.68 0.967422
Target:  5'- -gCCAaugcauuCGGCGCAAUcAUUGCgGCUCGu -3'
miRNA:   3'- uaGGU-------GCCGUGUUAcUAACGgUGAGC- -5'
23554 5' -52.2 NC_005260.1 + 71440 0.69 0.964514
Target:  5'- --aCGCGGauuaGCAAUGug-GCCACUCa -3'
miRNA:   3'- uagGUGCCg---UGUUACuaaCGGUGAGc -5'
23554 5' -52.2 NC_005260.1 + 116518 0.7 0.939955
Target:  5'- uAUUCACGGUaaaaaacgaagcgGCAAaGAUacUGCCGCUCGu -3'
miRNA:   3'- -UAGGUGCCG-------------UGUUaCUA--ACGGUGAGC- -5'
23554 5' -52.2 NC_005260.1 + 229814 0.71 0.90772
Target:  5'- -aUCACGGCgaaauggcuGCGAUGGaUGCCGCUUGc -3'
miRNA:   3'- uaGGUGCCG---------UGUUACUaACGGUGAGC- -5'
23554 5' -52.2 NC_005260.1 + 228353 0.71 0.90772
Target:  5'- ----uUGGCAUAGUGAUUGUCGCUUGu -3'
miRNA:   3'- uagguGCCGUGUUACUAACGGUGAGC- -5'
23554 5' -52.2 NC_005260.1 + 130541 0.71 0.90772
Target:  5'- uUCCgACGGCACGAUGAUUGUguUUg- -3'
miRNA:   3'- uAGG-UGCCGUGUUACUAACGguGAgc -5'
23554 5' -52.2 NC_005260.1 + 228130 1.08 0.010752
Target:  5'- aAUCCACGGCACAAUGAUUGCCACUCGa -3'
miRNA:   3'- -UAGGUGCCGUGUUACUAACGGUGAGC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.