miRNA: 3'- uaaCGGua-CGGUaUGUGCCGGCAGAa -5'
miRNA: 3'- uaaCGGUa-CGGUaUGUGC-----CGGCAGAA- -5'
miRNA: 3'- uaaCGGUACGGUau--GUGCCG-GCAGAA- -5'
miRNA: 3'- uaACGGUaCGGU--------------AUGUGCCGGCa-GAa -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23562 | 3' | -54.8 | NC_005261.1 | + | 132834 | 0.67 | 0.850472 |
|
Target: 5'- --cGCUuucGUCG-ACGCGGCCGUCUa -3' miRNA: 3'- uaaCGGua-CGGUaUGUGCCGGCAGAa -5' |
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| 23562 | 3' | -54.8 | NC_005261.1 | + | 135223 | 0.67 | 0.833578 |
|
Target: 5'- --gGCCAcgGCCGaGCGCGucgccGCCGUCUUu -3' miRNA: 3'- uaaCGGUa-CGGUaUGUGC-----CGGCAGAA- -5' |
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| 23562 | 3' | -54.8 | NC_005261.1 | + | 136360 | 0.72 | 0.549052 |
|
Target: 5'- --cGCCGUGCUGgccuCGCGGCgCGUCUUc -3' miRNA: 3'- uaaCGGUACGGUau--GUGCCG-GCAGAA- -5' |
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| 23562 | 3' | -54.8 | NC_005261.1 | + | 136738 | 0.67 | 0.847991 |
|
Target: 5'- -cUGCCG-GCCGcggcgguggccuucUGCGCGGCCGcgCUg -3' miRNA: 3'- uaACGGUaCGGU--------------AUGUGCCGGCa-GAa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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