Results 61 - 64 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23562 | 3' | -54.8 | NC_005261.1 | + | 120251 | 0.73 | 0.518125 |
Target: 5'- --cGCCGcggcUGCCAcGC-CGGCCGUCUa -3' miRNA: 3'- uaaCGGU----ACGGUaUGuGCCGGCAGAa -5' |
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23562 | 3' | -54.8 | NC_005261.1 | + | 101094 | 0.73 | 0.487914 |
Target: 5'- -gUGCCAUGCCAcucggUGcCACGGCCGgcgCg- -3' miRNA: 3'- uaACGGUACGGU-----AU-GUGCCGGCa--Gaa -5' |
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23562 | 3' | -54.8 | NC_005261.1 | + | 63553 | 0.74 | 0.478027 |
Target: 5'- --cGCCGUGCCcgcugGCGCGGCCGg--- -3' miRNA: 3'- uaaCGGUACGGua---UGUGCCGGCagaa -5' |
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23562 | 3' | -54.8 | NC_005261.1 | + | 128971 | 1.04 | 0.005427 |
Target: 5'- uAUUGCCAUGCCAUACACGGCCGUCUUg -3' miRNA: 3'- -UAACGGUACGGUAUGUGCCGGCAGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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