miRNA: 3'- aCA--AGCGGUCC-UCGCGCAucc-GCAac -5'
miRNA: 3'- acaaGCGGUCCucGCGCauCCGCaac -5'
miRNA: 3'- acaaGCGGUC-CuCGCGCaUCCGCAac -5'
miRNA: 3'- acaAG-CGGU--CC-UCGCGCAUCCGCaac -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23625 | 5' | -56.3 | NC_005261.1 | + | 18434 | 0.66 | 0.878331 |
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Target: 5'- cGUccUCGCCGGGcAGCGCGUucccCGUg- -3' miRNA: 3'- aCA--AGCGGUCC-UCGCGCAucc-GCAac -5' |
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| 23625 | 5' | -56.3 | NC_005261.1 | + | 23465 | 0.66 | 0.88541 |
|
Target: 5'- ---gCGCCAGGccCGCGgcGGCGa-- -3' miRNA: 3'- acaaGCGGUCCucGCGCauCCGCaac -5' |
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| 23625 | 5' | -56.3 | NC_005261.1 | + | 39992 | 0.66 | 0.88541 |
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Target: 5'- ---cCGCCGGcGuGCGUGaAGGCGUa- -3' miRNA: 3'- acaaGCGGUC-CuCGCGCaUCCGCAac -5' |
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| 23625 | 5' | -56.3 | NC_005261.1 | + | 50987 | 0.66 | 0.88541 |
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Target: 5'- --cUCgGCCGacGGcGGCGCGUGGGCGc-- -3' miRNA: 3'- acaAG-CGGU--CC-UCGCGCAUCCGCaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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