Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2373 | 3' | -58.5 | NC_001416.1 | + | 9258 | 0.66 | 0.508079 |
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Target: 5'- cGGGCAUccacgccaGCGUgaaGCUgguaucuccGGCAGAuuucuGCCCCUg -3' miRNA: 3'- -UCCGUG--------CGCA---CGA---------CCGUCUu----UGGGGG- -5' |
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| 2373 | 3' | -58.5 | NC_001416.1 | + | 33009 | 0.67 | 0.437981 |
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Target: 5'- cGGGCGaGC-UGCUGGUAGuGACgCgCCCa -3' miRNA: 3'- -UCCGUgCGcACGACCGUCuUUG-G-GGG- -5' |
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| 2373 | 3' | -58.5 | NC_001416.1 | + | 1362 | 0.67 | 0.418983 |
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Target: 5'- gAGGCACcugucagauugaGCGUGCaGcCAGuGAAUCCCCg -3' miRNA: 3'- -UCCGUG------------CGCACGaCcGUC-UUUGGGGG- -5' |
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| 2373 | 3' | -58.5 | NC_001416.1 | + | 5975 | 0.67 | 0.40875 |
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Target: 5'- cGGCACGUuccguuugaggauGUGCucUGGcCGGAGGCUgCCa -3' miRNA: 3'- uCCGUGCG-------------CACG--ACC-GUCUUUGGgGG- -5' |
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| 2373 | 3' | -58.5 | NC_001416.1 | + | 5598 | 0.69 | 0.348256 |
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Target: 5'- -aGCACGCGUGCUGuucuucGCGuccguGAGCCUCCu -3' miRNA: 3'- ucCGUGCGCACGAC------CGUc----UUUGGGGG- -5' |
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| 2373 | 3' | -58.5 | NC_001416.1 | + | 21442 | 0.7 | 0.279388 |
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Target: 5'- -aGCACGaccGCUGGCGGGuuUCCCCu -3' miRNA: 3'- ucCGUGCgcaCGACCGUCUuuGGGGG- -5' |
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| 2373 | 3' | -58.5 | NC_001416.1 | + | 16961 | 0.72 | 0.199623 |
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Target: 5'- cGGUgGCGUGUGCUGGCGGuGAACagccagaCCCg -3' miRNA: 3'- uCCG-UGCGCACGACCGUC-UUUGg------GGG- -5' |
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| 2373 | 3' | -58.5 | NC_001416.1 | + | 5538 | 1.12 | 0.000233 |
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Target: 5'- cAGGCACGCGUGCUGGCAGAAACCCCCg -3' miRNA: 3'- -UCCGUGCGCACGACCGUCUUUGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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