Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2374 | 3' | -56.6 | NC_001416.1 | + | 40472 | 0.66 | 0.619831 |
Target: 5'- aGUGUCGCCagagUGCCGcGAUGAaacGGGUa-- -3' miRNA: 3'- aCGCAGCGG----ACGGC-CUACU---UCUAggc -5' |
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2374 | 3' | -56.6 | NC_001416.1 | + | 39948 | 0.68 | 0.460761 |
Target: 5'- aGCGagGCCUGuauCCGGAUGcGGAgucuuaUCCGu -3' miRNA: 3'- aCGCagCGGAC---GGCCUACuUCU------AGGC- -5' |
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2374 | 3' | -56.6 | NC_001416.1 | + | 19301 | 0.68 | 0.458771 |
Target: 5'- gUGCGUaCGCCaugGCCGGAgUGGcucacagucgguGGUCCGg -3' miRNA: 3'- -ACGCA-GCGGa--CGGCCU-ACUu-----------CUAGGC- -5' |
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2374 | 3' | -56.6 | NC_001416.1 | + | 13178 | 0.71 | 0.342091 |
Target: 5'- aGCGagCGCCUGCCGGGcUGAAug-CCa -3' miRNA: 3'- aCGCa-GCGGACGGCCU-ACUUcuaGGc -5' |
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2374 | 3' | -56.6 | NC_001416.1 | + | 6396 | 1.08 | 0.000785 |
Target: 5'- cUGCGUCGCCUGCCGGAUGAAGAUCCGc -3' miRNA: 3'- -ACGCAGCGGACGGCCUACUUCUAGGC- -5' |
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2374 | 3' | -56.6 | NC_001416.1 | + | 3573 | 0.68 | 0.51083 |
Target: 5'- gGCG-CGCCcGCCGGGUaacucacGggGuAUCCa -3' miRNA: 3'- aCGCaGCGGaCGGCCUA-------CuuC-UAGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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