Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23801 | 5' | -57.5 | NC_005261.1 | + | 64519 | 0.74 | 0.381139 |
Target: 5'- -gCGGGCGGCuccuCGUCGGGGggcaGGUCcgGGa -3' miRNA: 3'- aaGCCCGCCG----GCAGUUCC----UCAGuaCC- -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 22696 | 0.73 | 0.415033 |
Target: 5'- -aCGGGCccggcgacgaGGCCGUCGAGGAGcCGgaGGa -3' miRNA: 3'- aaGCCCG----------CCGGCAGUUCCUCaGUa-CC- -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 105003 | 0.72 | 0.516918 |
Target: 5'- -gCGGGCGGCgGUCGGGGcGcCA-GGg -3' miRNA: 3'- aaGCCCGCCGgCAGUUCCuCaGUaCC- -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 23727 | 0.71 | 0.556459 |
Target: 5'- -gCGGGUGGCCGcgcCGAGGcgcGUCcgGGa -3' miRNA: 3'- aaGCCCGCCGGCa--GUUCCu--CAGuaCC- -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 23641 | 0.7 | 0.627394 |
Target: 5'- gUCGGGCGGCUGgaAGGGAGagG-GGa -3' miRNA: 3'- aAGCCCGCCGGCagUUCCUCagUaCC- -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 135808 | 0.69 | 0.688406 |
Target: 5'- -gUGGGCGacgcGCCGuUCGAGGAGUaCAucccUGGg -3' miRNA: 3'- aaGCCCGC----CGGC-AGUUCCUCA-GU----ACC- -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 55295 | 0.68 | 0.697459 |
Target: 5'- --gGGGCGcugaagaGCCGgugCGGGGGGUCAgGGg -3' miRNA: 3'- aagCCCGC-------CGGCa--GUUCCUCAGUaCC- -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 17866 | 0.68 | 0.708463 |
Target: 5'- -gCGGcCGGCCGgcaggcCAAGGGG-CAUGGu -3' miRNA: 3'- aaGCCcGCCGGCa-----GUUCCUCaGUACC- -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 84953 | 0.68 | 0.708463 |
Target: 5'- cUC-GGCGGCCGcCGAGGcGUCG-GGc -3' miRNA: 3'- aAGcCCGCCGGCaGUUCCuCAGUaCC- -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 98474 | 0.68 | 0.708463 |
Target: 5'- -aCGGGCGGCCGUCcuccgcGGGcUCGggcgGGu -3' miRNA: 3'- aaGCCCGCCGGCAGuu----CCUcAGUa---CC- -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 133185 | 0.68 | 0.728262 |
Target: 5'- -cCGcGGC-GCCGUCGAGGAGUuCAUc- -3' miRNA: 3'- aaGC-CCGcCGGCAGUUCCUCA-GUAcc -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 90238 | 0.68 | 0.737069 |
Target: 5'- -gCGGGCGcGCCGUCGgguaucaGGGcgccaaGGUCcUGGa -3' miRNA: 3'- aaGCCCGC-CGGCAGU-------UCC------UCAGuACC- -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 127020 | 0.68 | 0.738043 |
Target: 5'- --gGcGGCGGCCcgagCAGGGAGUCGUc- -3' miRNA: 3'- aagC-CCGCCGGca--GUUCCUCAGUAcc -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 80052 | 0.68 | 0.738043 |
Target: 5'- gUCGccacuGGCGGCgGgcgCGGGGGG-CGUGGg -3' miRNA: 3'- aAGC-----CCGCCGgCa--GUUCCUCaGUACC- -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 135663 | 0.68 | 0.738043 |
Target: 5'- -gCGGGCGGCCcucgcUCGAGGAGcUCu--- -3' miRNA: 3'- aaGCCCGCCGGc----AGUUCCUC-AGuacc -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 110182 | 0.68 | 0.747732 |
Target: 5'- -cCGGGCGGCaguuccgcgaCGUUGAGGAGgCcUGGc -3' miRNA: 3'- aaGCCCGCCG----------GCAGUUCCUCaGuACC- -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 73843 | 0.68 | 0.75158 |
Target: 5'- cUUGGGCGaGUCGgcgccccggaggcuuUUAAGcGGGUCGUGGg -3' miRNA: 3'- aAGCCCGC-CGGC---------------AGUUC-CUCAGUACC- -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 35309 | 0.67 | 0.75732 |
Target: 5'- -cCGGGCcgGGCUGcUCGGGGAGgCcgGGc -3' miRNA: 3'- aaGCCCG--CCGGC-AGUUCCUCaGuaCC- -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 33819 | 0.67 | 0.776156 |
Target: 5'- cUUUGGGCGGCCG---GGGGG-CGcGGg -3' miRNA: 3'- -AAGCCCGCCGGCaguUCCUCaGUaCC- -5' |
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23801 | 5' | -57.5 | NC_005261.1 | + | 98567 | 0.67 | 0.794481 |
Target: 5'- -gCGGGCGGCCGUCcuccgcgggcucGGGcGAGUa--GGc -3' miRNA: 3'- aaGCCCGCCGGCAG------------UUC-CUCAguaCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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