Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23805 | 3' | -51.6 | NC_005261.1 | + | 134285 | 0.76 | 0.63675 |
Target: 5'- aGAUCUGgGCGugGGUCcacGUCGCCGccuUCGc -3' miRNA: 3'- -CUAGAUgUGCugCCAG---UAGUGGC---AGC- -5' |
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23805 | 3' | -51.6 | NC_005261.1 | + | 53770 | 0.75 | 0.67857 |
Target: 5'- ----cGCGCGGCaaacgaccgGGUCGUCGCCGUCGc -3' miRNA: 3'- cuagaUGUGCUG---------CCAGUAGUGGCAGC- -5' |
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23805 | 3' | -51.6 | NC_005261.1 | + | 90228 | 0.72 | 0.85784 |
Target: 5'- ----cGCGCGGCGGgCGggcgCGCCGUCGg -3' miRNA: 3'- cuagaUGUGCUGCCaGUa---GUGGCAGC- -5' |
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23805 | 3' | -51.6 | NC_005261.1 | + | 81303 | 0.7 | 0.923631 |
Target: 5'- ----cGCACGuccgugccguugucGgGGUCGUCGCCGUCGu -3' miRNA: 3'- cuagaUGUGC--------------UgCCAGUAGUGGCAGC- -5' |
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23805 | 3' | -51.6 | NC_005261.1 | + | 49737 | 0.7 | 0.9253 |
Target: 5'- uGGUCgaagcugagGC-CGACGGUgAUgACCGUCGu -3' miRNA: 3'- -CUAGa--------UGuGCUGCCAgUAgUGGCAGC- -5' |
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23805 | 3' | -51.6 | NC_005261.1 | + | 68057 | 0.69 | 0.945394 |
Target: 5'- uGUCUACGCGggcACGGUgGUCuCgGUCGc -3' miRNA: 3'- cUAGAUGUGC---UGCCAgUAGuGgCAGC- -5' |
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23805 | 3' | -51.6 | NC_005261.1 | + | 62100 | 0.68 | 0.961566 |
Target: 5'- --gCUGCACGAUGG-CGUuggccaCGCCGUCc -3' miRNA: 3'- cuaGAUGUGCUGCCaGUA------GUGGCAGc -5' |
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23805 | 3' | -51.6 | NC_005261.1 | + | 2592 | 0.68 | 0.965022 |
Target: 5'- ----cACGCGGCGGaaGcCGCCGUCGg -3' miRNA: 3'- cuagaUGUGCUGCCagUaGUGGCAGC- -5' |
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23805 | 3' | -51.6 | NC_005261.1 | + | 15577 | 0.68 | 0.965022 |
Target: 5'- cGGUCgccuguaGCAUGACGaagcaGUCGCCGUCGa -3' miRNA: 3'- -CUAGa------UGUGCUGCcag--UAGUGGCAGC- -5' |
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23805 | 3' | -51.6 | NC_005261.1 | + | 81807 | 0.68 | 0.971269 |
Target: 5'- cGAgccgCUGCGCGGCGGUgacgccCcgCGCCGcgCGg -3' miRNA: 3'- -CUa---GAUGUGCUGCCA------GuaGUGGCa-GC- -5' |
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23805 | 3' | -51.6 | NC_005261.1 | + | 80007 | 0.67 | 0.980642 |
Target: 5'- cGAgCU-CACGGUGGUCAcccgcuuguuggcgUCGCCGUCGc -3' miRNA: 3'- -CUaGAuGUGCUGCCAGU--------------AGUGGCAGC- -5' |
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23805 | 3' | -51.6 | NC_005261.1 | + | 75709 | 0.67 | 0.981287 |
Target: 5'- --gCUGCGCGccACGGUCGacgccaUCACCG-CGc -3' miRNA: 3'- cuaGAUGUGC--UGCCAGU------AGUGGCaGC- -5' |
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23805 | 3' | -51.6 | NC_005261.1 | + | 50039 | 0.67 | 0.981287 |
Target: 5'- ----cGCGCGACGGUgCAgCGCCGcCGc -3' miRNA: 3'- cuagaUGUGCUGCCA-GUaGUGGCaGC- -5' |
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23805 | 3' | -51.6 | NC_005261.1 | + | 59903 | 0.66 | 0.985182 |
Target: 5'- cGUCgUAgGCGAagagcaGGUCGacgagcUCGCCGUCGg -3' miRNA: 3'- cUAG-AUgUGCUg-----CCAGU------AGUGGCAGC- -5' |
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23805 | 3' | -51.6 | NC_005261.1 | + | 63401 | 0.66 | 0.988427 |
Target: 5'- uGGUCgaACAUGA-GGUCGUCGgCGUCc -3' miRNA: 3'- -CUAGa-UGUGCUgCCAGUAGUgGCAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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