miRNA display CGI


Results 1 - 15 of 15 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23805 3' -51.6 NC_005261.1 + 134285 0.76 0.63675
Target:  5'- aGAUCUGgGCGugGGUCcacGUCGCCGccuUCGc -3'
miRNA:   3'- -CUAGAUgUGCugCCAG---UAGUGGC---AGC- -5'
23805 3' -51.6 NC_005261.1 + 53770 0.75 0.67857
Target:  5'- ----cGCGCGGCaaacgaccgGGUCGUCGCCGUCGc -3'
miRNA:   3'- cuagaUGUGCUG---------CCAGUAGUGGCAGC- -5'
23805 3' -51.6 NC_005261.1 + 90228 0.72 0.85784
Target:  5'- ----cGCGCGGCGGgCGggcgCGCCGUCGg -3'
miRNA:   3'- cuagaUGUGCUGCCaGUa---GUGGCAGC- -5'
23805 3' -51.6 NC_005261.1 + 81303 0.7 0.923631
Target:  5'- ----cGCACGuccgugccguugucGgGGUCGUCGCCGUCGu -3'
miRNA:   3'- cuagaUGUGC--------------UgCCAGUAGUGGCAGC- -5'
23805 3' -51.6 NC_005261.1 + 49737 0.7 0.9253
Target:  5'- uGGUCgaagcugagGC-CGACGGUgAUgACCGUCGu -3'
miRNA:   3'- -CUAGa--------UGuGCUGCCAgUAgUGGCAGC- -5'
23805 3' -51.6 NC_005261.1 + 68057 0.69 0.945394
Target:  5'- uGUCUACGCGggcACGGUgGUCuCgGUCGc -3'
miRNA:   3'- cUAGAUGUGC---UGCCAgUAGuGgCAGC- -5'
23805 3' -51.6 NC_005261.1 + 62100 0.68 0.961566
Target:  5'- --gCUGCACGAUGG-CGUuggccaCGCCGUCc -3'
miRNA:   3'- cuaGAUGUGCUGCCaGUA------GUGGCAGc -5'
23805 3' -51.6 NC_005261.1 + 2592 0.68 0.965022
Target:  5'- ----cACGCGGCGGaaGcCGCCGUCGg -3'
miRNA:   3'- cuagaUGUGCUGCCagUaGUGGCAGC- -5'
23805 3' -51.6 NC_005261.1 + 15577 0.68 0.965022
Target:  5'- cGGUCgccuguaGCAUGACGaagcaGUCGCCGUCGa -3'
miRNA:   3'- -CUAGa------UGUGCUGCcag--UAGUGGCAGC- -5'
23805 3' -51.6 NC_005261.1 + 81807 0.68 0.971269
Target:  5'- cGAgccgCUGCGCGGCGGUgacgccCcgCGCCGcgCGg -3'
miRNA:   3'- -CUa---GAUGUGCUGCCA------GuaGUGGCa-GC- -5'
23805 3' -51.6 NC_005261.1 + 80007 0.67 0.980642
Target:  5'- cGAgCU-CACGGUGGUCAcccgcuuguuggcgUCGCCGUCGc -3'
miRNA:   3'- -CUaGAuGUGCUGCCAGU--------------AGUGGCAGC- -5'
23805 3' -51.6 NC_005261.1 + 75709 0.67 0.981287
Target:  5'- --gCUGCGCGccACGGUCGacgccaUCACCG-CGc -3'
miRNA:   3'- cuaGAUGUGC--UGCCAGU------AGUGGCaGC- -5'
23805 3' -51.6 NC_005261.1 + 50039 0.67 0.981287
Target:  5'- ----cGCGCGACGGUgCAgCGCCGcCGc -3'
miRNA:   3'- cuagaUGUGCUGCCA-GUaGUGGCaGC- -5'
23805 3' -51.6 NC_005261.1 + 59903 0.66 0.985182
Target:  5'- cGUCgUAgGCGAagagcaGGUCGacgagcUCGCCGUCGg -3'
miRNA:   3'- cUAG-AUgUGCUg-----CCAGU------AGUGGCAGC- -5'
23805 3' -51.6 NC_005261.1 + 63401 0.66 0.988427
Target:  5'- uGGUCgaACAUGA-GGUCGUCGgCGUCc -3'
miRNA:   3'- -CUAGa-UGUGCUgCCAGUAGUgGCAGc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.