Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23807 | 3' | -56.3 | NC_005261.1 | + | 17082 | 0.66 | 0.894468 |
Target: 5'- gACG-GGAuGGGCCCgGUG-GUGugccAGCGCu -3' miRNA: 3'- -UGCaCCU-UCCGGGaCAUgCAC----UCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 94754 | 0.66 | 0.894468 |
Target: 5'- gACGUGGAGaucuGGUUCgaggACGUG-GCGCc -3' miRNA: 3'- -UGCACCUU----CCGGGaca-UGCACuCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 121812 | 0.66 | 0.894468 |
Target: 5'- cGCGUGGcuGGGCUCUGcgggccgccGCGgcGGCGCa -3' miRNA: 3'- -UGCACCu-UCCGGGACa--------UGCacUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 115585 | 0.66 | 0.887732 |
Target: 5'- gGCGaGGAAcuGGCC--GUACGUGAGCcccaGCg -3' miRNA: 3'- -UGCaCCUU--CCGGgaCAUGCACUCG----CG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 74422 | 0.66 | 0.880769 |
Target: 5'- gGCG-GGAGGGCCgaGga---GAGCGCc -3' miRNA: 3'- -UGCaCCUUCCGGgaCaugcaCUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 11828 | 0.66 | 0.880769 |
Target: 5'- -aGUGGGggcggGGGCCCcgcgGgcgcGCGUucgcGAGCGCg -3' miRNA: 3'- ugCACCU-----UCCGGGa---Ca---UGCA----CUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 15971 | 0.66 | 0.873583 |
Target: 5'- gGCGggGGAGGGCCCgGgcCGcucacccggGcAGCGCg -3' miRNA: 3'- -UGCa-CCUUCCGGGaCauGCa--------C-UCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 123655 | 0.66 | 0.873583 |
Target: 5'- cACGcccaGGAAGGCCgCUGUcaucucgcgcACGUcccGGCGCu -3' miRNA: 3'- -UGCa---CCUUCCGG-GACA----------UGCAc--UCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 44436 | 0.66 | 0.858566 |
Target: 5'- gACGUGGAGcGGCUCUcgcGgcUGUGGGaGCa -3' miRNA: 3'- -UGCACCUU-CCGGGA---CauGCACUCgCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 49280 | 0.67 | 0.850745 |
Target: 5'- cGCGaGGAAGgcgcagaaGCCCgcgGCgGUGAGCGCc -3' miRNA: 3'- -UGCaCCUUC--------CGGGacaUG-CACUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 100290 | 0.67 | 0.834513 |
Target: 5'- aGCGgcaGAAGGCCCcaaaGUccGCGUcgcGGGCGCg -3' miRNA: 3'- -UGCac-CUUCCGGGa---CA--UGCA---CUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 25280 | 0.67 | 0.834513 |
Target: 5'- cGCGaGGggGcGCggUCUGUGgcCGUGAGCGUc -3' miRNA: 3'- -UGCaCCuuC-CG--GGACAU--GCACUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 97482 | 0.67 | 0.834513 |
Target: 5'- cCGUcgGGggGGCCC--UugG-GGGCGCc -3' miRNA: 3'- uGCA--CCuuCCGGGacAugCaCUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 135081 | 0.67 | 0.826115 |
Target: 5'- gGCGgcgGGcucgcGGCCCg--GCGgcgGAGCGCg -3' miRNA: 3'- -UGCa--CCuu---CCGGGacaUGCa--CUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 52250 | 0.67 | 0.81754 |
Target: 5'- uCGUgGGAGGGCgCUGUccGCGccgGGGgGCg -3' miRNA: 3'- uGCA-CCUUCCGgGACA--UGCa--CUCgCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 111092 | 0.68 | 0.808796 |
Target: 5'- gUGUGGggGGCCggggaCUGggUG-GAGUGCu -3' miRNA: 3'- uGCACCuuCCGG-----GACauGCaCUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 124188 | 0.68 | 0.808796 |
Target: 5'- cGCGUGGggGGacgcgUCCgGUgGCGUGAacuGCGUg -3' miRNA: 3'- -UGCACCuuCC-----GGGaCA-UGCACU---CGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 28762 | 0.68 | 0.808796 |
Target: 5'- cGCGgccGggGGCgCCUGggccgcgGCGcGGGCGCu -3' miRNA: 3'- -UGCac-CuuCCG-GGACa------UGCaCUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 40226 | 0.68 | 0.79989 |
Target: 5'- aGCGUcGucgucucuGGCCCgggGgcCGUGAGCGCc -3' miRNA: 3'- -UGCAcCuu------CCGGGa--CauGCACUCGCG- -5' |
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23807 | 3' | -56.3 | NC_005261.1 | + | 43786 | 0.68 | 0.790832 |
Target: 5'- cGCGcGGAGGGCgCCgcggGUGaggGGGCGCc -3' miRNA: 3'- -UGCaCCUUCCG-GGa---CAUgcaCUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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