Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23808 | 3' | -54.4 | NC_005261.1 | + | 4528 | 0.68 | 0.874605 |
Target: 5'- cGGGCGCCGcggCGAGggcgcCGGGCccUGGCCu -3' miRNA: 3'- -CUUGCGGCa--GCUCaa---GCUCG--ACUGGu -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 16294 | 0.69 | 0.809175 |
Target: 5'- gGGGCGCCGUCGGGcccagCG-GC-GGCCGg -3' miRNA: 3'- -CUUGCGGCAGCUCaa---GCuCGaCUGGU- -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 22709 | 0.67 | 0.908479 |
Target: 5'- cGAgGCCGUCGAG---GAGCcggagGACCAg -3' miRNA: 3'- cUUgCGGCAGCUCaagCUCGa----CUGGU- -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 23556 | 0.69 | 0.809175 |
Target: 5'- ---aGCCGUCGcaGGUggggcaGGGCUGGCCGu -3' miRNA: 3'- cuugCGGCAGC--UCAag----CUCGACUGGU- -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 27795 | 0.72 | 0.672234 |
Target: 5'- --uUGCCGUCGAG--CGAGCUaGCCAg -3' miRNA: 3'- cuuGCGGCAGCUCaaGCUCGAcUGGU- -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 28588 | 0.7 | 0.800167 |
Target: 5'- cGAGCGCCGgCGGGccgcccgcgcCGAGCUGGCgGa -3' miRNA: 3'- -CUUGCGGCaGCUCaa--------GCUCGACUGgU- -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 30889 | 0.66 | 0.936317 |
Target: 5'- --cUGCCGccCGAGcUCGGGCUGggGCCGc -3' miRNA: 3'- cuuGCGGCa-GCUCaAGCUCGAC--UGGU- -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 31443 | 0.66 | 0.929671 |
Target: 5'- cGGCGCCGcccggCGAGgaCGAGCgccggcggccugagUGGCCGc -3' miRNA: 3'- cUUGCGGCa----GCUCaaGCUCG--------------ACUGGU- -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 31651 | 0.69 | 0.809175 |
Target: 5'- cGGCGCgGUCGAGUaccucugccugCG-GCUGGCCGc -3' miRNA: 3'- cUUGCGgCAGCUCAa----------GCuCGACUGGU- -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 34298 | 0.69 | 0.818018 |
Target: 5'- gGGACGCCGggagCGAGaccgaCGGGgaGGCCGa -3' miRNA: 3'- -CUUGCGGCa---GCUCaa---GCUCgaCUGGU- -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 35303 | 0.71 | 0.703017 |
Target: 5'- aGAGCGCCGggcCGGGcugcUCGGGgaGGCCGg -3' miRNA: 3'- -CUUGCGGCa--GCUCa---AGCUCgaCUGGU- -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 40676 | 0.72 | 0.682541 |
Target: 5'- cGACGCCGUCGGGgagcCGGcGCcGGCCGg -3' miRNA: 3'- cUUGCGGCAGCUCaa--GCU-CGaCUGGU- -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 44870 | 0.67 | 0.895635 |
Target: 5'- aGAGCGCCGccgCGAGcgccgCGGGgaUGGCCGg -3' miRNA: 3'- -CUUGCGGCa--GCUCaa---GCUCg-ACUGGU- -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 44968 | 0.66 | 0.92592 |
Target: 5'- gGGACGCgCG-CGAGggccCGGGCgGGCCGc -3' miRNA: 3'- -CUUGCG-GCaGCUCaa--GCUCGaCUGGU- -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 53881 | 0.72 | 0.692805 |
Target: 5'- -cGCGCUGUCGAG-UCGAaGCgGGCCc -3' miRNA: 3'- cuUGCGGCAGCUCaAGCU-CGaCUGGu -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 58560 | 0.67 | 0.919208 |
Target: 5'- -uGCGCCGUCGuGgccgcgagcggCGAGCgcgaGACCu -3' miRNA: 3'- cuUGCGGCAGCuCaa---------GCUCGa---CUGGu -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 68098 | 0.68 | 0.888856 |
Target: 5'- gGGAgGCCGUgaAGUUCGGGCUGgaGCUg -3' miRNA: 3'- -CUUgCGGCAgcUCAAGCUCGAC--UGGu -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 68898 | 0.72 | 0.682541 |
Target: 5'- cGGACGCCGUCGuccugaaGAGC-GACCAc -3' miRNA: 3'- -CUUGCGGCAGCucaag--CUCGaCUGGU- -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 71676 | 0.69 | 0.809175 |
Target: 5'- -uGCGCaCGUCGGcGUcugCGGGCgUGACCAu -3' miRNA: 3'- cuUGCG-GCAGCU-CAa--GCUCG-ACUGGU- -5' |
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23808 | 3' | -54.4 | NC_005261.1 | + | 72464 | 0.72 | 0.672234 |
Target: 5'- cGAGCGCgG-CGAGcgCGAGCgcGGCCAg -3' miRNA: 3'- -CUUGCGgCaGCUCaaGCUCGa-CUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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