Results 21 - 40 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23812 | 3' | -58.3 | NC_005261.1 | + | 14134 | 0.67 | 0.741947 |
Target: 5'- gGUGGGCagucgCuCGCGAGG-ACG-CUCCAg -3' miRNA: 3'- gCGCUCGa----G-GCGCUCCaUGCaGAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 14019 | 0.67 | 0.75147 |
Target: 5'- gGUGAGCUCUGCGAuGGccaGCGgggCCAu -3' miRNA: 3'- gCGCUCGAGGCGCU-CCa--UGCagaGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 23192 | 0.67 | 0.75147 |
Target: 5'- aGCGAGCgcgCCGCGGGGcccgcagcgGCGg--CCGc -3' miRNA: 3'- gCGCUCGa--GGCGCUCCa--------UGCagaGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 85560 | 0.67 | 0.767421 |
Target: 5'- uGCG-GCgCCGCGAGGUucggcgccgcgaccGCGgcCUCCGc -3' miRNA: 3'- gCGCuCGaGGCGCUCCA--------------UGCa-GAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 28433 | 0.67 | 0.770201 |
Target: 5'- cCGCGcGCaCCGCGGccGGccaugGCGUCUCCc -3' miRNA: 3'- -GCGCuCGaGGCGCU--CCa----UGCAGAGGu -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 95210 | 0.67 | 0.770201 |
Target: 5'- cCGCccGC-CCGCGcGGUGCGUgUUCCAg -3' miRNA: 3'- -GCGcuCGaGGCGCuCCAUGCA-GAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 106823 | 0.67 | 0.770201 |
Target: 5'- cCGCcAGCagCGCGuGGUgcGCGUCgUCCAg -3' miRNA: 3'- -GCGcUCGagGCGCuCCA--UGCAG-AGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 93925 | 0.67 | 0.779393 |
Target: 5'- cCGCGGGCuugcgccgUCCGCGGGcgcCGcCUCCGg -3' miRNA: 3'- -GCGCUCG--------AGGCGCUCcauGCaGAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 135100 | 0.66 | 0.797384 |
Target: 5'- gGCG-GCggagCGCGAGGcggaccccGCGUCUCCGc -3' miRNA: 3'- gCGCuCGag--GCGCUCCa-------UGCAGAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 79429 | 0.66 | 0.797384 |
Target: 5'- gGCGAGCUCgGCGcgcGuGUACacCUCCAg -3' miRNA: 3'- gCGCUCGAGgCGCu--C-CAUGcaGAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 47097 | 0.66 | 0.797384 |
Target: 5'- gGCGGGCcagagcccgUCCGUGAGG-ACGUgcgcgUCCAg -3' miRNA: 3'- gCGCUCG---------AGGCGCUCCaUGCAg----AGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 22047 | 0.66 | 0.797384 |
Target: 5'- --gGGGCUCCgGCGGGGUucaGgggCUCCGg -3' miRNA: 3'- gcgCUCGAGG-CGCUCCAug-Ca--GAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 70347 | 0.66 | 0.797384 |
Target: 5'- aGCGGGCcggggaCCGgGAGGcggcGCGcCUCCAc -3' miRNA: 3'- gCGCUCGa-----GGCgCUCCa---UGCaGAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 55043 | 0.66 | 0.806167 |
Target: 5'- cCGCGcAGCUgCGCGGGGccgGCGagCUCg- -3' miRNA: 3'- -GCGC-UCGAgGCGCUCCa--UGCa-GAGgu -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 65590 | 0.66 | 0.809638 |
Target: 5'- cCGCGAGCUCCugcucgccggggucgGCGAcGUACG-CgCCGa -3' miRNA: 3'- -GCGCUCGAGG---------------CGCUcCAUGCaGaGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 46660 | 0.66 | 0.814797 |
Target: 5'- gGCGaAGUUCCGCGcguuGGccGCGcgCUCCAc -3' miRNA: 3'- gCGC-UCGAGGCGCu---CCa-UGCa-GAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 104075 | 0.66 | 0.823266 |
Target: 5'- gCGCGuccAGCUCgGCGgacAGGgcgGCGgcgCUCCGc -3' miRNA: 3'- -GCGC---UCGAGgCGC---UCCa--UGCa--GAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 4185 | 0.66 | 0.823266 |
Target: 5'- cCGCGAGCUUCGCGcucugcagccAGGccaugGCGUCg--- -3' miRNA: 3'- -GCGCUCGAGGCGC----------UCCa----UGCAGaggu -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 99597 | 0.66 | 0.831567 |
Target: 5'- aGCGAGUccaCCGCGA---GCGUCgUCCAc -3' miRNA: 3'- gCGCUCGa--GGCGCUccaUGCAG-AGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 57294 | 0.66 | 0.831567 |
Target: 5'- uCGCGAGCagCCGCGcg--ACGUCcucgUCCAu -3' miRNA: 3'- -GCGCUCGa-GGCGCuccaUGCAG----AGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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