Results 21 - 40 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23812 | 3' | -58.3 | NC_005261.1 | + | 76648 | 0.69 | 0.612838 |
Target: 5'- aCGCGAGCgcCUGCGAGGU-CGgcgCCGu -3' miRNA: 3'- -GCGCUCGa-GGCGCUCCAuGCagaGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 79429 | 0.66 | 0.797384 |
Target: 5'- gGCGAGCUCgGCGcgcGuGUACacCUCCAg -3' miRNA: 3'- gCGCUCGAGgCGCu--C-CAUGcaGAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 80248 | 0.77 | 0.253499 |
Target: 5'- aCGaCGaAGCcgCCGCGcgccAGGUACGUCUCCAg -3' miRNA: 3'- -GC-GC-UCGa-GGCGC----UCCAUGCAGAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 81911 | 0.68 | 0.677176 |
Target: 5'- aCGCGgccAGCUCCuccuUGAGGUGCGggagcaggcccacguUCUCCAc -3' miRNA: 3'- -GCGC---UCGAGGc---GCUCCAUGC---------------AGAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 85560 | 0.67 | 0.767421 |
Target: 5'- uGCG-GCgCCGCGAGGUucggcgccgcgaccGCGgcCUCCGc -3' miRNA: 3'- gCGCuCGaGGCGCUCCA--------------UGCa-GAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 86198 | 0.7 | 0.572788 |
Target: 5'- aGCGGGCUCCGCGucGGcGCGcCgaaCCGg -3' miRNA: 3'- gCGCUCGAGGCGCu-CCaUGCaGa--GGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 86284 | 0.67 | 0.732331 |
Target: 5'- gCGCGAGCacagcCCGCaGGGcACGUCgcccUCCAg -3' miRNA: 3'- -GCGCUCGa----GGCGcUCCaUGCAG----AGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 93925 | 0.67 | 0.779393 |
Target: 5'- cCGCGGGCuugcgccgUCCGCGGGcgcCGcCUCCGg -3' miRNA: 3'- -GCGCUCG--------AGGCGCUCcauGCaGAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 95210 | 0.67 | 0.770201 |
Target: 5'- cCGCccGC-CCGCGcGGUGCGUgUUCCAg -3' miRNA: 3'- -GCGcuCGaGGCGCuCCAUGCA-GAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 98676 | 0.69 | 0.653107 |
Target: 5'- gGCGGGCUCCGCcggggccgGAGGcGCGg--CCGg -3' miRNA: 3'- gCGCUCGAGGCG--------CUCCaUGCagaGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 99597 | 0.66 | 0.831567 |
Target: 5'- aGCGAGUccaCCGCGA---GCGUCgUCCAc -3' miRNA: 3'- gCGCUCGa--GGCGCUccaUGCAG-AGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 104075 | 0.66 | 0.823266 |
Target: 5'- gCGCGuccAGCUCgGCGgacAGGgcgGCGgcgCUCCGc -3' miRNA: 3'- -GCGC---UCGAGgCGC---UCCa--UGCa--GAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 104171 | 0.68 | 0.692122 |
Target: 5'- cCGCGGGCgCCGCGcgcGGGU-CGUCgggcgggacggcgUCCAg -3' miRNA: 3'- -GCGCUCGaGGCGC---UCCAuGCAG-------------AGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 106823 | 0.67 | 0.770201 |
Target: 5'- cCGCcAGCagCGCGuGGUgcGCGUCgUCCAg -3' miRNA: 3'- -GCGcUCGagGCGCuCCA--UGCAG-AGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 109326 | 0.79 | 0.18981 |
Target: 5'- cCGCGAGCgcggCCGCGGGGUcggcggccGCGUCgccgUCCAg -3' miRNA: 3'- -GCGCUCGa---GGCGCUCCA--------UGCAG----AGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 120312 | 0.68 | 0.703014 |
Target: 5'- gGCGAGgCugUCUGCGAGGcgcgcUGCGUCcCCGa -3' miRNA: 3'- gCGCUC-G--AGGCGCUCC-----AUGCAGaGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 121656 | 0.68 | 0.711875 |
Target: 5'- gCGCGGGCugUCCGCGucggcguAGGUguagGCGccCUCCAg -3' miRNA: 3'- -GCGCUCG--AGGCGC-------UCCA----UGCa-GAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 123250 | 0.67 | 0.729429 |
Target: 5'- gCGCGGGCcCCGCccaucgcccgugacGAGGUGCGgguggUCCGc -3' miRNA: 3'- -GCGCUCGaGGCG--------------CUCCAUGCag---AGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 135100 | 0.66 | 0.797384 |
Target: 5'- gGCG-GCggagCGCGAGGcggaccccGCGUCUCCGc -3' miRNA: 3'- gCGCuCGag--GCGCUCCa-------UGCAGAGGU- -5' |
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23812 | 3' | -58.3 | NC_005261.1 | + | 135747 | 0.69 | 0.643045 |
Target: 5'- gGCGcGCcucagCCGCGcGGUGCG-CUCCGg -3' miRNA: 3'- gCGCuCGa----GGCGCuCCAUGCaGAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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