Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23822 | 5' | -59.9 | NC_005261.1 | + | 40231 | 0.66 | 0.699389 |
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Target: 5'- --cGucGUCuCUGGCCCGGGGgccgUGAGCGc -3' miRNA: 3'- cauCccCAG-GACCGGGUCCU----ACUCGU- -5' |
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| 23822 | 5' | -59.9 | NC_005261.1 | + | 25997 | 0.66 | 0.689379 |
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Target: 5'- -gAGGGGUgg-GGcCCCGGGAgagagaGAGCAa -3' miRNA: 3'- caUCCCCAggaCC-GGGUCCUa-----CUCGU- -5' |
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| 23822 | 5' | -59.9 | NC_005261.1 | + | 48311 | 0.66 | 0.679323 |
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Target: 5'- -cGGGGGgcgcgUCgGGCCCGGGcucggGGGCGc -3' miRNA: 3'- caUCCCCa----GGaCCGGGUCCua---CUCGU- -5' |
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| 23822 | 5' | -59.9 | NC_005261.1 | + | 1069 | 0.67 | 0.64896 |
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Target: 5'- -cGGGGGg-CUGGCCCcgcuccAGGgcGAGCc -3' miRNA: 3'- caUCCCCagGACCGGG------UCCuaCUCGu -5' |
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| 23822 | 5' | -59.9 | NC_005261.1 | + | 121194 | 0.67 | 0.628637 |
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Target: 5'- --cGGGG-CCgGGCCCGGGGccgcGGGCc -3' miRNA: 3'- cauCCCCaGGaCCGGGUCCUa---CUCGu -5' |
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| 23822 | 5' | -59.9 | NC_005261.1 | + | 16170 | 0.67 | 0.628637 |
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Target: 5'- aGUAGGGGgcgcgggCgUGG-CgGGGGUGGGCGg -3' miRNA: 3'- -CAUCCCCa------GgACCgGgUCCUACUCGU- -5' |
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| 23822 | 5' | -59.9 | NC_005261.1 | + | 29119 | 0.67 | 0.598192 |
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Target: 5'- -gAGGGGagcCCUGGCgCCGGG--GGGCGc -3' miRNA: 3'- caUCCCCa--GGACCG-GGUCCuaCUCGU- -5' |
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| 23822 | 5' | -59.9 | NC_005261.1 | + | 46047 | 0.69 | 0.499261 |
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Target: 5'- gGUAGaGGGUCCagGGCagCGGGAaGGGCGc -3' miRNA: 3'- -CAUC-CCCAGGa-CCGg-GUCCUaCUCGU- -5' |
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| 23822 | 5' | -59.9 | NC_005261.1 | + | 15932 | 0.69 | 0.499261 |
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Target: 5'- --cGGGG-CCggGGCCCGGGcagGAGCc -3' miRNA: 3'- cauCCCCaGGa-CCGGGUCCua-CUCGu -5' |
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| 23822 | 5' | -59.9 | NC_005261.1 | + | 24288 | 0.72 | 0.358882 |
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Target: 5'- gGUGGGGGUgggGGgCCAGGAUGAGgGu -3' miRNA: 3'- -CAUCCCCAggaCCgGGUCCUACUCgU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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