Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23833 | 3' | -54.2 | NC_005261.1 | + | 98988 | 0.67 | 0.94027 |
Target: 5'- --aCGUCGUCCUCGgcaaucuucagCGUCGcGAGc-- -3' miRNA: 3'- ggaGCAGCAGGAGCa----------GCAGC-CUCaac -5' |
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23833 | 3' | -54.2 | NC_005261.1 | + | 81325 | 0.67 | 0.944807 |
Target: 5'- ---gGUCGUCgC-CGUCGUCGGGGc-- -3' miRNA: 3'- ggagCAGCAG-GaGCAGCAGCCUCaac -5' |
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23833 | 3' | -54.2 | NC_005261.1 | + | 124866 | 0.67 | 0.949112 |
Target: 5'- gCgUCGUCGcCUUCGUCGUCuGGcccgGGUUc -3' miRNA: 3'- -GgAGCAGCaGGAGCAGCAG-CC----UCAAc -5' |
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23833 | 3' | -54.2 | NC_005261.1 | + | 34822 | 0.66 | 0.956663 |
Target: 5'- uCCUgGUCGgagUCggaggagUCGUCGUCGGGGc-- -3' miRNA: 3'- -GGAgCAGCa--GG-------AGCAGCAGCCUCaac -5' |
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23833 | 3' | -54.2 | NC_005261.1 | + | 125250 | 0.66 | 0.960667 |
Target: 5'- uUCUCGUCcucacugguGUCCUCGUC--CGGGGg-- -3' miRNA: 3'- -GGAGCAG---------CAGGAGCAGcaGCCUCaac -5' |
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23833 | 3' | -54.2 | NC_005261.1 | + | 102573 | 0.66 | 0.967276 |
Target: 5'- gCCUCGcCGUCCgCGUCGgCGGc---- -3' miRNA: 3'- -GGAGCaGCAGGaGCAGCaGCCucaac -5' |
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23833 | 3' | -54.2 | NC_005261.1 | + | 121522 | 0.66 | 0.967276 |
Target: 5'- --cCGUCGauggcgagcUCCUCGUCGcugUCGGAGc-- -3' miRNA: 3'- ggaGCAGC---------AGGAGCAGC---AGCCUCaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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