miRNA display CGI


Results 21 - 27 of 27 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
23833 3' -54.2 NC_005261.1 + 98988 0.67 0.94027
Target:  5'- --aCGUCGUCCUCGgcaaucuucagCGUCGcGAGc-- -3'
miRNA:   3'- ggaGCAGCAGGAGCa----------GCAGC-CUCaac -5'
23833 3' -54.2 NC_005261.1 + 81325 0.67 0.944807
Target:  5'- ---gGUCGUCgC-CGUCGUCGGGGc-- -3'
miRNA:   3'- ggagCAGCAG-GaGCAGCAGCCUCaac -5'
23833 3' -54.2 NC_005261.1 + 124866 0.67 0.949112
Target:  5'- gCgUCGUCGcCUUCGUCGUCuGGcccgGGUUc -3'
miRNA:   3'- -GgAGCAGCaGGAGCAGCAG-CC----UCAAc -5'
23833 3' -54.2 NC_005261.1 + 34822 0.66 0.956663
Target:  5'- uCCUgGUCGgagUCggaggagUCGUCGUCGGGGc-- -3'
miRNA:   3'- -GGAgCAGCa--GG-------AGCAGCAGCCUCaac -5'
23833 3' -54.2 NC_005261.1 + 125250 0.66 0.960667
Target:  5'- uUCUCGUCcucacugguGUCCUCGUC--CGGGGg-- -3'
miRNA:   3'- -GGAGCAG---------CAGGAGCAGcaGCCUCaac -5'
23833 3' -54.2 NC_005261.1 + 102573 0.66 0.967276
Target:  5'- gCCUCGcCGUCCgCGUCGgCGGc---- -3'
miRNA:   3'- -GGAGCaGCAGGaGCAGCaGCCucaac -5'
23833 3' -54.2 NC_005261.1 + 121522 0.66 0.967276
Target:  5'- --cCGUCGauggcgagcUCCUCGUCGcugUCGGAGc-- -3'
miRNA:   3'- ggaGCAGC---------AGGAGCAGC---AGCCUCaac -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.