Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23833 | 3' | -54.2 | NC_005261.1 | + | 108670 | 0.71 | 0.79336 |
Target: 5'- gCgUCG-CGUCCUCGUCGuccggcUCGGGGa-- -3' miRNA: 3'- -GgAGCaGCAGGAGCAGC------AGCCUCaac -5' |
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23833 | 3' | -54.2 | NC_005261.1 | + | 23090 | 0.71 | 0.811079 |
Target: 5'- uCCUCGuagUCGUCCUCGuagUCGUCcucgcugcuGGAGUc- -3' miRNA: 3'- -GGAGC---AGCAGGAGC---AGCAG---------CCUCAac -5' |
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23833 | 3' | -54.2 | NC_005261.1 | + | 112728 | 0.71 | 0.811079 |
Target: 5'- uCCUCGUCGUCCgcagcaUCGUCaugaCGGGGa-- -3' miRNA: 3'- -GGAGCAGCAGG------AGCAGca--GCCUCaac -5' |
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23833 | 3' | -54.2 | NC_005261.1 | + | 86099 | 0.7 | 0.819702 |
Target: 5'- uUCUCGUCGgcgCC-CGggCGUCGGAGc-- -3' miRNA: 3'- -GGAGCAGCa--GGaGCa-GCAGCCUCaac -5' |
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23833 | 3' | -54.2 | NC_005261.1 | + | 23069 | 0.69 | 0.867645 |
Target: 5'- uCUUCGUCGUCCUCGUaGUCGn----- -3' miRNA: 3'- -GGAGCAGCAGGAGCAgCAGCcucaac -5' |
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23833 | 3' | -54.2 | NC_005261.1 | + | 53154 | 0.69 | 0.867645 |
Target: 5'- --cCGUCGcgCCgcCGUCGUCGGGGUc- -3' miRNA: 3'- ggaGCAGCa-GGa-GCAGCAGCCUCAac -5' |
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23833 | 3' | -54.2 | NC_005261.1 | + | 121522 | 0.66 | 0.967276 |
Target: 5'- --cCGUCGauggcgagcUCCUCGUCGcugUCGGAGc-- -3' miRNA: 3'- ggaGCAGC---------AGGAGCAGC---AGCCUCaac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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