Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23836 | 5' | -53 | NC_005261.1 | + | 70647 | 0.66 | 0.957102 |
Target: 5'- -gCGGCgcugAGGUUcGgcGCCG-CGGCCGCg -3' miRNA: 3'- aaGUCG----UCCAA-CauCGGCaGUUGGUG- -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 66027 | 0.66 | 0.957102 |
Target: 5'- -cCGGCGcGUcGUAGUCGgCGGCCACg -3' miRNA: 3'- aaGUCGUcCAaCAUCGGCaGUUGGUG- -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 627 | 0.66 | 0.957102 |
Target: 5'- ---cGCAGGggaUGUGGCgG-CGGCCGCc -3' miRNA: 3'- aaguCGUCCa--ACAUCGgCaGUUGGUG- -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 33941 | 0.66 | 0.957102 |
Target: 5'- -cCAGCAGGaUGgcGCCGcCugUCGCu -3' miRNA: 3'- aaGUCGUCCaACauCGGCaGuuGGUG- -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 136808 | 0.66 | 0.957102 |
Target: 5'- --uGGCGGGcucGUAGCCGUaGACgGCg -3' miRNA: 3'- aagUCGUCCaa-CAUCGGCAgUUGgUG- -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 121770 | 0.66 | 0.953033 |
Target: 5'- --gAGCGcGGUgggGUGGCCG-CGGCCGg -3' miRNA: 3'- aagUCGU-CCAa--CAUCGGCaGUUGGUg -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 96956 | 0.66 | 0.953033 |
Target: 5'- aUCGGCGGGgc--AGCCGcCGGgCGCg -3' miRNA: 3'- aAGUCGUCCaacaUCGGCaGUUgGUG- -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 84133 | 0.66 | 0.953033 |
Target: 5'- --gGGCGGGgucgcUGgcgGGCC-UCGGCCACg -3' miRNA: 3'- aagUCGUCCa----ACa--UCGGcAGUUGGUG- -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 107291 | 0.66 | 0.948712 |
Target: 5'- -gCGGCAGGcacuccGCCG-CGGCCACg -3' miRNA: 3'- aaGUCGUCCaacau-CGGCaGUUGGUG- -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 122140 | 0.66 | 0.944134 |
Target: 5'- -gCAGguGGUgGUgcacGGCCGacgccagcUCGACCGCg -3' miRNA: 3'- aaGUCguCCAaCA----UCGGC--------AGUUGGUG- -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 62141 | 0.66 | 0.944134 |
Target: 5'- gUCAGCAGGUUGgGGa--UCAGCgACa -3' miRNA: 3'- aAGUCGUCCAACaUCggcAGUUGgUG- -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 38612 | 0.66 | 0.944134 |
Target: 5'- --uGGCGGGUguUGGCCG-CGGCCGa -3' miRNA: 3'- aagUCGUCCAacAUCGGCaGUUGGUg -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 122250 | 0.66 | 0.93262 |
Target: 5'- aUCAGCAGGgccagcgccgccGCCGUCAggGCCGu -3' miRNA: 3'- aAGUCGUCCaacau-------CGGCAGU--UGGUg -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 82716 | 0.67 | 0.923217 |
Target: 5'- --gAGCAGGcaGUccacGCCGUCcAGCCGCg -3' miRNA: 3'- aagUCGUCCaaCAu---CGGCAG-UUGGUG- -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 8504 | 0.67 | 0.91733 |
Target: 5'- -cCAGCGGGgg--GGCCGgcCAAUCGCa -3' miRNA: 3'- aaGUCGUCCaacaUCGGCa-GUUGGUG- -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 39974 | 0.67 | 0.91118 |
Target: 5'- cUCAGCGGGUUGcgcgcGCCGcCGGCgUGCg -3' miRNA: 3'- aAGUCGUCCAACau---CGGCaGUUG-GUG- -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 80379 | 0.67 | 0.91118 |
Target: 5'- gUUCuGCAGcGcgaaagUGUGGCCGUCGcUCACa -3' miRNA: 3'- -AAGuCGUC-Ca-----ACAUCGGCAGUuGGUG- -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 109345 | 0.67 | 0.91118 |
Target: 5'- gUCGGCGGccgcGUcGCCGUcCAGCCGCu -3' miRNA: 3'- aAGUCGUCcaa-CAuCGGCA-GUUGGUG- -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 98564 | 0.67 | 0.91118 |
Target: 5'- -cCGGCGGGc---GGCCGUCcuCCGCg -3' miRNA: 3'- aaGUCGUCCaacaUCGGCAGuuGGUG- -5' |
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23836 | 5' | -53 | NC_005261.1 | + | 133081 | 0.67 | 0.910551 |
Target: 5'- gUCAGCuucuucgagcgcaAGGcc---GCCGUCAACCGCg -3' miRNA: 3'- aAGUCG-------------UCCaacauCGGCAGUUGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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