miRNA: 3'- -AUGCaGAUGAaGGA----CCCGa---UGCCGGG- -5'
miRNA: 3'- auGCAGa-UGaa---GGACCCGAugCCGGG- -5'
miRNA: 3'- aUGCAGAUGaaGGACCCGaUGCCG-GG- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23841 | 5' | -55.9 | NC_005261.1 | + | 135641 | 0.75 | 0.39039 |
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Target: 5'- aUGCG-CUGCUaCCUgcgcGGGCgcggGCGGCCCu -3' miRNA: 3'- -AUGCaGAUGAaGGA----CCCGa---UGCCGGG- -5' |
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| 23841 | 5' | -55.9 | NC_005261.1 | + | 127898 | 0.76 | 0.365484 |
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Target: 5'- -cCGUCggGCccgggCCUGGGCUugGGCUCg -3' miRNA: 3'- auGCAGa-UGaa---GGACCCGAugCCGGG- -5' |
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| 23841 | 5' | -55.9 | NC_005261.1 | + | 118814 | 0.79 | 0.234024 |
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Target: 5'- gGCGUCUGCgcCCUGGGC-GCGGCaCUg -3' miRNA: 3'- aUGCAGAUGaaGGACCCGaUGCCG-GG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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