Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23848 | 3' | -52.6 | NC_005261.1 | + | 15497 | 0.72 | 0.764216 |
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Target: 5'- cUCG-CUGCGg--GCGGGGCGUGUGUCg -3' miRNA: 3'- -AGCaGGCGUagaUGCUCUGCACGUAG- -5' |
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| 23848 | 3' | -52.6 | NC_005261.1 | + | 71681 | 0.73 | 0.724404 |
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Target: 5'- aCGUCgGCGUCUGCG-GGCGUGacCAUg -3' miRNA: 3'- aGCAGgCGUAGAUGCuCUGCAC--GUAg -5' |
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| 23848 | 3' | -52.6 | NC_005261.1 | + | 104167 | 0.73 | 0.7183 |
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Target: 5'- cCGUCCGCgggcgccgcgcgcggGUCgucggGCGGGACG-GCGUCc -3' miRNA: 3'- aGCAGGCG---------------UAGa----UGCUCUGCaCGUAG- -5' |
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| 23848 | 3' | -52.6 | NC_005261.1 | + | 126789 | 0.75 | 0.620365 |
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Target: 5'- gCGUCUGCGUCUGcCGAGagcaGCGcgGCGUCu -3' miRNA: 3'- aGCAGGCGUAGAU-GCUC----UGCa-CGUAG- -5' |
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| 23848 | 3' | -52.6 | NC_005261.1 | + | 77129 | 0.76 | 0.561919 |
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Target: 5'- cUCGUCCGCgacaagugggacaccGUCUcgcgccgccgccGCGAGGCGgGCAUCg -3' miRNA: 3'- -AGCAGGCG---------------UAGA------------UGCUCUGCaCGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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