Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
23856 | 5' | -60.5 | NC_005261.1 | + | 7687 | 0.66 | 0.681337 |
Target: 5'- gUGUUCUUCUUCUucugcauucuugCUCUCUCUCUCC-Cg -3' miRNA: 3'- -ACGAGGAGGGGA------------GAGGGAGAGAGGaG- -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 16356 | 0.66 | 0.680338 |
Target: 5'- gGCUCCguccucuUCCCCgccgCCUUCUCcgugggCCUCg -3' miRNA: 3'- aCGAGG-------AGGGGaga-GGGAGAGa-----GGAG- -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 735 | 0.66 | 0.671334 |
Target: 5'- -cCUCCUCCgCggUCCCUcCUCUCC-Cg -3' miRNA: 3'- acGAGGAGGgGagAGGGA-GAGAGGaG- -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 124726 | 0.66 | 0.6613 |
Target: 5'- aGCUCCUCCaCCacgCUCC-UCUCcCCg- -3' miRNA: 3'- aCGAGGAGG-GGa--GAGGgAGAGaGGag -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 9702 | 0.66 | 0.6613 |
Target: 5'- cGCUCCgcCCCCUC-CCCUagcagcagcgCUCCg- -3' miRNA: 3'- aCGAGGa-GGGGAGaGGGAga--------GAGGag -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 120756 | 0.67 | 0.580856 |
Target: 5'- -cUUCCUuuUCCCUCcCCCUCUUUCCa- -3' miRNA: 3'- acGAGGA--GGGGAGaGGGAGAGAGGag -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 52093 | 0.67 | 0.580856 |
Target: 5'- -cCUCCcCCCUUCcCCCcCUCUCCUg -3' miRNA: 3'- acGAGGaGGGGAGaGGGaGAGAGGAg -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 77862 | 0.69 | 0.51217 |
Target: 5'- gGCUCCacaCgCCCUCUCCaggCUCgcguccgccgUCUCCUCg -3' miRNA: 3'- aCGAGGa--G-GGGAGAGG---GAG----------AGAGGAG- -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 76830 | 0.69 | 0.493136 |
Target: 5'- aGCaacuaCCUgCCCUCgUCUCgCUCUCCUCg -3' miRNA: 3'- aCGa----GGAgGGGAG-AGGGaGAGAGGAG- -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 2853 | 0.69 | 0.493136 |
Target: 5'- gGCggC-CCCCUCgUCCCUUUCUuCCUCc -3' miRNA: 3'- aCGagGaGGGGAG-AGGGAGAGA-GGAG- -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 22374 | 0.69 | 0.490309 |
Target: 5'- -uCUCCUCCCUgccCUCCCUCcccacccccaccuaUCUCCa- -3' miRNA: 3'- acGAGGAGGGGa--GAGGGAG--------------AGAGGag -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 6016 | 0.69 | 0.483741 |
Target: 5'- gGCUCCggcgagcgUCCCCcCUUCUUCuUCUCCUUc -3' miRNA: 3'- aCGAGG--------AGGGGaGAGGGAG-AGAGGAG- -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 11133 | 0.69 | 0.483741 |
Target: 5'- gUGCgccgCCUCCgCCgcUCUCCCcCUCcCCUCc -3' miRNA: 3'- -ACGa---GGAGG-GG--AGAGGGaGAGaGGAG- -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 28325 | 0.69 | 0.483741 |
Target: 5'- cGCUCucgCUCUCC-CUCCCUCcCUcCCUCc -3' miRNA: 3'- aCGAG---GAGGGGaGAGGGAGaGA-GGAG- -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 26581 | 0.7 | 0.456093 |
Target: 5'- -cCUCCUCCUC-CUCCU--UCUCCUCu -3' miRNA: 3'- acGAGGAGGGGaGAGGGagAGAGGAG- -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 26410 | 0.7 | 0.447067 |
Target: 5'- cGCUCuCUCUCCcC-CCUUCUCUcCCUCu -3' miRNA: 3'- aCGAG-GAGGGGaGaGGGAGAGA-GGAG- -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 10716 | 0.72 | 0.339637 |
Target: 5'- cGcCUCCgggccCCCCUCUUCCUCcgccgcCUCCUCc -3' miRNA: 3'- aC-GAGGa----GGGGAGAGGGAGa-----GAGGAG- -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 120785 | 0.72 | 0.317656 |
Target: 5'- ---gCCUCCCUUCUCCUUCUcCUCCa- -3' miRNA: 3'- acgaGGAGGGGAGAGGGAGA-GAGGag -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 28395 | 0.73 | 0.303606 |
Target: 5'- cGCcUCUCCCCUaccCCCUC-CUCCUCc -3' miRNA: 3'- aCGaGGAGGGGAga-GGGAGaGAGGAG- -5' |
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23856 | 5' | -60.5 | NC_005261.1 | + | 6945 | 0.73 | 0.283436 |
Target: 5'- gGCUCCUCCUCUCUgCCUUUUUUUUa -3' miRNA: 3'- aCGAGGAGGGGAGAgGGAGAGAGGAg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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