Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 23870 | 5' | -57 | NC_005262.1 | + | 45880 | 0.68 | 0.527882 |
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Target: 5'- uGGC-GUCGGC--GCCgAUCGGCGGCu -3' miRNA: 3'- cCUGaCAGCCGgaUGG-UAGUCGCCGu -5' |
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| 23870 | 5' | -57 | NC_005262.1 | + | 33661 | 0.68 | 0.527882 |
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Target: 5'- cGGCgcuUCGGCg-ACCAgCAGCGGCAc -3' miRNA: 3'- cCUGac-AGCCGgaUGGUaGUCGCCGU- -5' |
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| 23870 | 5' | -57 | NC_005262.1 | + | 47737 | 0.68 | 0.53831 |
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Target: 5'- aGGAUcGUCGGCCgcaaGUCgcgguAGCGGCGg -3' miRNA: 3'- -CCUGaCAGCCGGauggUAG-----UCGCCGU- -5' |
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| 23870 | 5' | -57 | NC_005262.1 | + | 11895 | 0.68 | 0.559369 |
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Target: 5'- cGGCgaagcgGUCGGU--GCCGuUCGGCGGCGu -3' miRNA: 3'- cCUGa-----CAGCCGgaUGGU-AGUCGCCGU- -5' |
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| 23870 | 5' | -57 | NC_005262.1 | + | 46407 | 0.66 | 0.634429 |
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Target: 5'- cGGcAUUGUCGGCC-GCCGagUCGacCGGCAg -3' miRNA: 3'- -CC-UGACAGCCGGaUGGU--AGUc-GCCGU- -5' |
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| 23870 | 5' | -57 | NC_005262.1 | + | 31763 | 0.66 | 0.666741 |
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Target: 5'- cGGGUUuUCGGCCUGCauCAUC-GCGGCc -3' miRNA: 3'- -CCUGAcAGCCGGAUG--GUAGuCGCCGu -5' |
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| 23870 | 5' | -57 | NC_005262.1 | + | 17158 | 0.66 | 0.677463 |
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Target: 5'- -cGCUGUCGGCC----AUCAGCGuGCc -3' miRNA: 3'- ccUGACAGCCGGauggUAGUCGC-CGu -5' |
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| 23870 | 5' | -57 | NC_005262.1 | + | 49618 | 0.66 | 0.677463 |
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Target: 5'- cGGAUggagCGGCUUGCCGcacuccUCGgcGCGGCGg -3' miRNA: 3'- -CCUGaca-GCCGGAUGGU------AGU--CGCCGU- -5' |
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| 23870 | 5' | -57 | NC_005262.1 | + | 59937 | 1.1 | 0.000698 |
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Target: 5'- gGGACUGUCGGCCUACCAUCAGCGGCAc -3' miRNA: 3'- -CCUGACAGCCGGAUGGUAGUCGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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