Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23870 | 5' | -57 | NC_005262.1 | + | 46407 | 0.66 | 0.634429 |
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Target: 5'- cGGcAUUGUCGGCC-GCCGagUCGacCGGCAg -3' miRNA: 3'- -CC-UGACAGCCGGaUGGU--AGUc-GCCGU- -5' |
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| 23870 | 5' | -57 | NC_005262.1 | + | 33888 | 0.66 | 0.634429 |
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Target: 5'- aGACgaucgugcGUCGGCCggcgGCCcgaucggUAGCGGCGg -3' miRNA: 3'- cCUGa-------CAGCCGGa---UGGua-----GUCGCCGU- -5' |
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| 23870 | 5' | -57 | NC_005262.1 | + | 37006 | 0.66 | 0.645214 |
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Target: 5'- ----cGUCGGCgaGacggaCAUCGGCGGCGg -3' miRNA: 3'- ccugaCAGCCGgaUg----GUAGUCGCCGU- -5' |
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| 23870 | 5' | -57 | NC_005262.1 | + | 48366 | 0.66 | 0.645214 |
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Target: 5'- -uGCUGgCGGCCgGCUAUCAGuCGGg- -3' miRNA: 3'- ccUGACaGCCGGaUGGUAGUC-GCCgu -5' |
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| 23870 | 5' | -57 | NC_005262.1 | + | 7627 | 0.66 | 0.655988 |
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Target: 5'- cGGAC-GUUGGCCgaaugucgaCGcCGGCGGCGa -3' miRNA: 3'- -CCUGaCAGCCGGaug------GUaGUCGCCGU- -5' |
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| 23870 | 5' | -57 | NC_005262.1 | + | 31763 | 0.66 | 0.666741 |
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Target: 5'- cGGGUUuUCGGCCUGCauCAUC-GCGGCc -3' miRNA: 3'- -CCUGAcAGCCGGAUG--GUAGuCGCCGu -5' |
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| 23870 | 5' | -57 | NC_005262.1 | + | 5049 | 0.66 | 0.666741 |
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Target: 5'- cGGcgcCUGaUCGGCgggcagGCCcgCGGCGGCGg -3' miRNA: 3'- -CCu--GAC-AGCCGga----UGGuaGUCGCCGU- -5' |
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| 23870 | 5' | -57 | NC_005262.1 | + | 17158 | 0.66 | 0.677463 |
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Target: 5'- -cGCUGUCGGCC----AUCAGCGuGCc -3' miRNA: 3'- ccUGACAGCCGGauggUAGUCGC-CGu -5' |
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| 23870 | 5' | -57 | NC_005262.1 | + | 49618 | 0.66 | 0.677463 |
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Target: 5'- cGGAUggagCGGCUUGCCGcacuccUCGgcGCGGCGg -3' miRNA: 3'- -CCUGaca-GCCGGAUGGU------AGU--CGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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