Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2389 | 3' | -51.7 | NC_001416.1 | + | 40276 | 1.12 | 0.001122 |
Target: 5'- aCGCAUAAUAGCUUCUGUGCGCCGGACg -3' miRNA: 3'- -GCGUAUUAUCGAAGACACGCGGCCUG- -5' |
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2389 | 3' | -51.7 | NC_001416.1 | + | 1167 | 0.72 | 0.496205 |
Target: 5'- gGCGU---GGCUUCUG-GUGCCuGGGCg -3' miRNA: 3'- gCGUAuuaUCGAAGACaCGCGG-CCUG- -5' |
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2389 | 3' | -51.7 | NC_001416.1 | + | 40710 | 0.68 | 0.755373 |
Target: 5'- uGCGUGaAUAGCguuuugCUGaUGUGCUGGAg -3' miRNA: 3'- gCGUAU-UAUCGaa----GAC-ACGCGGCCUg -5' |
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2389 | 3' | -51.7 | NC_001416.1 | + | 20211 | 0.67 | 0.807271 |
Target: 5'- uGC-UGAUGccGCUUC--UGCGCCGGAg -3' miRNA: 3'- gCGuAUUAU--CGAAGacACGCGGCCUg -5' |
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2389 | 3' | -51.7 | NC_001416.1 | + | 7489 | 0.67 | 0.776635 |
Target: 5'- aCGCGggaugaaccuGUGGCaUUUGUGCuGCCGGGa -3' miRNA: 3'- -GCGUau--------UAUCGaAGACACG-CGGCCUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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