Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 23966 | 5' | -48.1 | NC_005262.1 | + | 13697 | 0.66 | 0.981023 |
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Target: 5'- cGGCuuCUGUUcGCGCGGCGcGggUUCGGUc -3' miRNA: 3'- -CCGc-GAUAA-UGUGUUGU-CuuAGGCCA- -5' |
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| 23966 | 5' | -48.1 | NC_005262.1 | + | 3733 | 0.66 | 0.981023 |
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Target: 5'- cGCGCggcGUUGCGCAGgAuGAAcgCCGGg -3' miRNA: 3'- cCGCGa--UAAUGUGUUgU-CUUa-GGCCa -5' |
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| 23966 | 5' | -48.1 | NC_005262.1 | + | 24763 | 0.66 | 0.983263 |
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Target: 5'- uGGCGCcGUUAC-CGAUgaGGAAgaugCCGGc -3' miRNA: 3'- -CCGCGaUAAUGuGUUG--UCUUa---GGCCa -5' |
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| 23966 | 5' | -48.1 | NC_005262.1 | + | 58840 | 0.66 | 0.983263 |
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Target: 5'- cGGCGCUucgGCAC-GCAGA--UCGGc -3' miRNA: 3'- -CCGCGAuaaUGUGuUGUCUuaGGCCa -5' |
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| 23966 | 5' | -48.1 | NC_005262.1 | + | 17331 | 0.66 | 0.987138 |
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Target: 5'- uGCGUcGUggccgGCGCGGCAuucGggUCCGGc -3' miRNA: 3'- cCGCGaUAa----UGUGUUGU---CuuAGGCCa -5' |
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| 23966 | 5' | -48.1 | NC_005262.1 | + | 3008 | 0.66 | 0.987138 |
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Target: 5'- cGGaaGCUGUUGCACAu---GAUCCGGc -3' miRNA: 3'- -CCg-CGAUAAUGUGUugucUUAGGCCa -5' |
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| 23966 | 5' | -48.1 | NC_005262.1 | + | 12175 | 0.66 | 0.988795 |
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Target: 5'- cGCGCUGUcUGCGCGGCAGGAg----- -3' miRNA: 3'- cCGCGAUA-AUGUGUUGUCUUaggcca -5' |
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| 23966 | 5' | -48.1 | NC_005262.1 | + | 41608 | 0.66 | 0.988795 |
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Target: 5'- uGGCGCUca-GgGCAGCGGAA--CGGUg -3' miRNA: 3'- -CCGCGAuaaUgUGUUGUCUUagGCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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