Results 21 - 31 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24076 | 3' | -55.9 | NC_005262.1 | + | 32151 | 0.68 | 0.566164 |
Target: 5'- gCGGGcGGCACG-UGGAUcgacuuccagAAGGCG-GCGg -3' miRNA: 3'- -GUUC-CCGUGCuACCUG----------UUCCGCuCGC- -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 16580 | 0.68 | 0.609388 |
Target: 5'- cCGAGcaGGCGCGugugcUGGuCGAGGCG-GCGa -3' miRNA: 3'- -GUUC--CCGUGCu----ACCuGUUCCGCuCGC- -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 59503 | 0.69 | 0.544833 |
Target: 5'- gAGGGGCGCG-UGauUGAGGCGGGUGc -3' miRNA: 3'- gUUCCCGUGCuACcuGUUCCGCUCGC- -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 36759 | 0.68 | 0.566164 |
Target: 5'- uGAGGu--CGAUGG-CGGGGCGGGCu -3' miRNA: 3'- gUUCCcguGCUACCuGUUCCGCUCGc -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 61065 | 0.68 | 0.566164 |
Target: 5'- --cGGGaccuCGA-GGcGCAGGGCGAGCGc -3' miRNA: 3'- guuCCCgu--GCUaCC-UGUUCCGCUCGC- -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 9682 | 0.68 | 0.598532 |
Target: 5'- gCGAGGGCGCcGUcGACGucgAGGCGcAGCa -3' miRNA: 3'- -GUUCCCGUGcUAcCUGU---UCCGC-UCGc -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 60318 | 0.67 | 0.630061 |
Target: 5'- --cGGGCGCGAUccgcaaugGGugAGGGaggucgaCGGGCGg -3' miRNA: 3'- guuCCCGUGCUA--------CCugUUCC-------GCUCGC- -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 45649 | 0.66 | 0.696136 |
Target: 5'- --cGGGCACGcgcucccGGuuGAGGCGcAGCGc -3' miRNA: 3'- guuCCCGUGCua-----CCugUUCCGC-UCGC- -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 34125 | 0.66 | 0.706817 |
Target: 5'- --cGGGUugGgcGGGCugcgcGGGcGCGGGCGg -3' miRNA: 3'- guuCCCGugCuaCCUG-----UUC-CGCUCGC- -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 43049 | 0.66 | 0.717426 |
Target: 5'- -uGGGGUuCGAccGGGCAGGGguCGAGCu -3' miRNA: 3'- guUCCCGuGCUa-CCUGUUCC--GCUCGc -5' |
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24076 | 3' | -55.9 | NC_005262.1 | + | 23819 | 0.73 | 0.335833 |
Target: 5'- gAGGGGUugGucaucucAUGGGCGccGGCGAGCGc -3' miRNA: 3'- gUUCCCGugC-------UACCUGUu-CCGCUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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