Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24132 | 3' | -54.9 | NC_005263.2 | + | 12519 | 0.71 | 0.427227 |
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Target: 5'- gGCaGUUUGCGcCCGGUCAgauuCUGaCGGCCg -3' miRNA: 3'- gCGaCAGACGU-GGCUAGU----GAC-GCUGG- -5' |
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| 24132 | 3' | -54.9 | NC_005263.2 | + | 17386 | 0.72 | 0.379848 |
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Target: 5'- aGCUGUCUGCcggcgcgGCCGcgCGCcugGCGGgCg -3' miRNA: 3'- gCGACAGACG-------UGGCuaGUGa--CGCUgG- -5' |
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| 24132 | 3' | -54.9 | NC_005263.2 | + | 21960 | 0.72 | 0.354522 |
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Target: 5'- gCGCUGUUUGCGCaguuGAUCGCcaacGuCGACCc -3' miRNA: 3'- -GCGACAGACGUGg---CUAGUGa---C-GCUGG- -5' |
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| 24132 | 3' | -54.9 | NC_005263.2 | + | 25062 | 0.72 | 0.34607 |
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Target: 5'- uGCUGUaCUGCuuCUGAaCGgUGCGGCCg -3' miRNA: 3'- gCGACA-GACGu-GGCUaGUgACGCUGG- -5' |
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| 24132 | 3' | -54.9 | NC_005263.2 | + | 6229 | 0.72 | 0.34607 |
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Target: 5'- aGCgga-UGC-CCGAUCGCUcGCGGCCa -3' miRNA: 3'- gCGacagACGuGGCUAGUGA-CGCUGG- -5' |
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| 24132 | 3' | -54.9 | NC_005263.2 | + | 20339 | 0.73 | 0.337762 |
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Target: 5'- cCGCUGUCgGCACCGAUac---CGGCCu -3' miRNA: 3'- -GCGACAGaCGUGGCUAgugacGCUGG- -5' |
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| 24132 | 3' | -54.9 | NC_005263.2 | + | 33979 | 0.79 | 0.137358 |
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Target: 5'- gCGCUGgccgCUGCGgcuaCGAUCGCgacgGCGACCg -3' miRNA: 3'- -GCGACa---GACGUg---GCUAGUGa---CGCUGG- -5' |
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| 24132 | 3' | -54.9 | NC_005263.2 | + | 39853 | 1.13 | 0.00054 |
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Target: 5'- uCGCUGUCUGCACCGAUCACUGCGACCa -3' miRNA: 3'- -GCGACAGACGUGGCUAGUGACGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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