Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24280 | 5' | -55.3 | NC_005263.2 | + | 19608 | 1.02 | 0.002099 |
Target: 5'- cUUCGUCGACAUCGCGCUCGAGCAGUUc -3' miRNA: 3'- -AAGCAGCUGUAGCGCGAGCUCGUCAA- -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 19465 | 0.77 | 0.151412 |
Target: 5'- cUCG-CGGCGUUccgcacgGCGCUCGAGCAGUa -3' miRNA: 3'- aAGCaGCUGUAG-------CGCGAGCUCGUCAa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 38449 | 0.72 | 0.298783 |
Target: 5'- cUCGgcggCGACAUUGCGCUCGA-CAGc- -3' miRNA: 3'- aAGCa---GCUGUAGCGCGAGCUcGUCaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 6609 | 0.72 | 0.314376 |
Target: 5'- aUCGcguUCGGCggCGCGCUCGGcGCGGUc -3' miRNA: 3'- aAGC---AGCUGuaGCGCGAGCU-CGUCAa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 28272 | 0.7 | 0.401382 |
Target: 5'- aUCGUCGAUcgCGCGCgacucaccUUGuGCAGUa -3' miRNA: 3'- aAGCAGCUGuaGCGCG--------AGCuCGUCAa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 27428 | 0.69 | 0.440221 |
Target: 5'- -cUGUCG-CGUCGCGCUCG-GCAa-- -3' miRNA: 3'- aaGCAGCuGUAGCGCGAGCuCGUcaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 10330 | 0.69 | 0.460425 |
Target: 5'- cUCGUCGAUcUCGCGCaggCGcGGCAGc- -3' miRNA: 3'- aAGCAGCUGuAGCGCGa--GC-UCGUCaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 3770 | 0.68 | 0.491621 |
Target: 5'- -aUGUCGACGUCGCGUUCuGGuCGGc- -3' miRNA: 3'- aaGCAGCUGUAGCGCGAGcUC-GUCaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 46172 | 0.68 | 0.502237 |
Target: 5'- -gCGUCGcCGgugccggCGCGCUCGuGCAGa- -3' miRNA: 3'- aaGCAGCuGUa------GCGCGAGCuCGUCaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 13837 | 0.68 | 0.512951 |
Target: 5'- cUUCGU--GCAUCGCGCgguacUCGGGCAGc- -3' miRNA: 3'- -AAGCAgcUGUAGCGCG-----AGCUCGUCaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 17395 | 0.68 | 0.523759 |
Target: 5'- -cCGgcgCGGCcgCGCGCcuggCGGGCGGUg -3' miRNA: 3'- aaGCa--GCUGuaGCGCGa---GCUCGUCAa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 913 | 0.67 | 0.534652 |
Target: 5'- gUUgGUCGGCAUCGCGUccUCGuAGCGu-- -3' miRNA: 3'- -AAgCAGCUGUAGCGCG--AGC-UCGUcaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 41086 | 0.67 | 0.554454 |
Target: 5'- aUCGUCGACAcguuUCGCGCcgccaauccgcaCGuGCGGUUu -3' miRNA: 3'- aAGCAGCUGU----AGCGCGa-----------GCuCGUCAA- -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 31924 | 0.67 | 0.556668 |
Target: 5'- gUUCGUCGACGauuUUGCGUUCGcGGCGu-- -3' miRNA: 3'- -AAGCAGCUGU---AGCGCGAGC-UCGUcaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 16596 | 0.67 | 0.578938 |
Target: 5'- gUUCGcCGGgcUCGCGCgCGAGCAGc- -3' miRNA: 3'- -AAGCaGCUguAGCGCGaGCUCGUCaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 4722 | 0.67 | 0.578938 |
Target: 5'- cUCGUCcggguACGUCGCGCccgCGAGCAc-- -3' miRNA: 3'- aAGCAGc----UGUAGCGCGa--GCUCGUcaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 17153 | 0.67 | 0.590147 |
Target: 5'- --gGUCGACGUCGCgguGUUCGgcuGGCAGa- -3' miRNA: 3'- aagCAGCUGUAGCG---CGAGC---UCGUCaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 18428 | 0.66 | 0.612666 |
Target: 5'- cUUCGUCGGCGaUGaacCGCUCGAGguGc- -3' miRNA: 3'- -AAGCAGCUGUaGC---GCGAGCUCguCaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 29307 | 0.66 | 0.612666 |
Target: 5'- --gGUCGGCAugcgcaUCGCGCgcggCGAGCAc-- -3' miRNA: 3'- aagCAGCUGU------AGCGCGa---GCUCGUcaa -5' |
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24280 | 5' | -55.3 | NC_005263.2 | + | 22552 | 0.66 | 0.635257 |
Target: 5'- -aCGcCGACGUCGCGCUCGcgaucaacucgaAGCu--- -3' miRNA: 3'- aaGCaGCUGUAGCGCGAGC------------UCGucaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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