Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24284 | 5' | -53.6 | NC_005263.2 | + | 3906 | 0.66 | 0.777186 |
Target: 5'- gUCGUgguagCGCCGGGcgGCAAUGGCgGCAGGc -3' miRNA: 3'- gAGUAg----GUGGUCC--UGUUGCUG-CGUCC- -5' |
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24284 | 5' | -53.6 | NC_005263.2 | + | 36598 | 0.66 | 0.777186 |
Target: 5'- uUCAUCgCgcgcgACCAGugcaacaaGGCGACGGCGCAGu -3' miRNA: 3'- gAGUAG-G-----UGGUC--------CUGUUGCUGCGUCc -5' |
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24284 | 5' | -53.6 | NC_005263.2 | + | 29066 | 0.66 | 0.787251 |
Target: 5'- -gCGUCC-UCGGGGCGGCG-CGCAucGGc -3' miRNA: 3'- gaGUAGGuGGUCCUGUUGCuGCGU--CC- -5' |
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24284 | 5' | -53.6 | NC_005263.2 | + | 35081 | 0.66 | 0.805907 |
Target: 5'- aUCGUgcuaguaCC-CCAGGAuuggcaauuauCGACGGCGCAGa -3' miRNA: 3'- gAGUA-------GGuGGUCCU-----------GUUGCUGCGUCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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