Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24332 | 5' | -57.4 | NC_005264.1 | + | 23054 | 0.66 | 0.890536 |
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Target: 5'- aCGCGUUCgugCuugCUuGGGGuaCCGGGAg -3' miRNA: 3'- -GCGCAAGa--Gua-GAuCCCUggGGCCCU- -5' |
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| 24332 | 5' | -57.4 | NC_005264.1 | + | 85832 | 0.66 | 0.883837 |
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Target: 5'- gCGCGUcaCUCAcgu-GGGACCCggaGGGAg -3' miRNA: 3'- -GCGCAa-GAGUagauCCCUGGGg--CCCU- -5' |
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| 24332 | 5' | -57.4 | NC_005264.1 | + | 139315 | 0.66 | 0.869803 |
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Target: 5'- uGCGUUCUCggGUCgacAGGG-CCguaCGGGGg -3' miRNA: 3'- gCGCAAGAG--UAGa--UCCCuGGg--GCCCU- -5' |
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| 24332 | 5' | -57.4 | NC_005264.1 | + | 124370 | 0.67 | 0.854952 |
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Target: 5'- aGCGgucgCUCGUCccGGGcGGCCUCGcGGAu -3' miRNA: 3'- gCGCaa--GAGUAGa-UCC-CUGGGGC-CCU- -5' |
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| 24332 | 5' | -57.4 | NC_005264.1 | + | 5343 | 0.67 | 0.854952 |
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Target: 5'- aGCGgucgCUCGUCccGGGcGGCCUCGcGGAu -3' miRNA: 3'- gCGCaa--GAGUAGa-UCC-CUGGGGC-CCU- -5' |
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| 24332 | 5' | -57.4 | NC_005264.1 | + | 25650 | 0.67 | 0.812859 |
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Target: 5'- cCGCGUUUUCcucaaucggcacGUCUGGGGGCagcagaagggaCGGGAc -3' miRNA: 3'- -GCGCAAGAG------------UAGAUCCCUGgg---------GCCCU- -5' |
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| 24332 | 5' | -57.4 | NC_005264.1 | + | 32909 | 0.69 | 0.742138 |
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Target: 5'- uGCGUUCUCgAUCgcgcAGGGGUCgCGGGGc -3' miRNA: 3'- gCGCAAGAG-UAGa---UCCCUGGgGCCCU- -5' |
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| 24332 | 5' | -57.4 | NC_005264.1 | + | 19247 | 0.73 | 0.487213 |
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Target: 5'- cCGCGccaUUCUCGUuccCUccGGGCCCCGGGGa -3' miRNA: 3'- -GCGC---AAGAGUA---GAucCCUGGGGCCCU- -5' |
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| 24332 | 5' | -57.4 | NC_005264.1 | + | 161309 | 1.09 | 0.002587 |
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Target: 5'- gCGCGUUCUCAUCUAGGGACCCCGGGAg -3' miRNA: 3'- -GCGCAAGAGUAGAUCCCUGGGGCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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