Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24346 | 5' | -54.5 | NC_005264.1 | + | 146477 | 1.11 | 0.003237 |
Target: 5'- uCCCCCCCAGCACUUUGCUUUCACCGAg -3' miRNA: 3'- -GGGGGGGUCGUGAAACGAAAGUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 60772 | 0.75 | 0.540444 |
Target: 5'- uUCCCCCCAGC-CU---CUUUCGCUGAa -3' miRNA: 3'- -GGGGGGGUCGuGAaacGAAAGUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 144331 | 0.72 | 0.723639 |
Target: 5'- gCCCCCgaUCGGCAaggcGUUUUCGCCGAa -3' miRNA: 3'- -GGGGG--GGUCGUgaaaCGAAAGUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 142378 | 0.71 | 0.733535 |
Target: 5'- gCCCUCCCAuGCGCgccGCUgucCGCCGGc -3' miRNA: 3'- -GGGGGGGU-CGUGaaaCGAaa-GUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 114231 | 0.71 | 0.743342 |
Target: 5'- aCCUCCCCGGCcuACgc-GUUUUCGCCa- -3' miRNA: 3'- -GGGGGGGUCG--UGaaaCGAAAGUGGcu -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 75275 | 0.71 | 0.743342 |
Target: 5'- uCCUCgUCGGCGCUUUGCUggu-CCGAu -3' miRNA: 3'- -GGGGgGGUCGUGAAACGAaaguGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 122052 | 0.71 | 0.762651 |
Target: 5'- gCCCCCC-GCAgUUUGCag-CGCCGc -3' miRNA: 3'- gGGGGGGuCGUgAAACGaaaGUGGCu -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 3025 | 0.71 | 0.762651 |
Target: 5'- gCCCCCC-GCAgUUUGCag-CGCCGc -3' miRNA: 3'- gGGGGGGuCGUgAAACGaaaGUGGCu -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 94448 | 0.7 | 0.790715 |
Target: 5'- uCCUCCCgCAGaGCgagucgUUGCUcUUCACCGAg -3' miRNA: 3'- -GGGGGG-GUCgUGa-----AACGA-AAGUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 107294 | 0.7 | 0.808718 |
Target: 5'- cCCCCUCCAGUACUUccg--UCGCCa- -3' miRNA: 3'- -GGGGGGGUCGUGAAacgaaAGUGGcu -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 146375 | 0.7 | 0.808718 |
Target: 5'- gCCCCCCAGCGCUgg---UUCgACCa- -3' miRNA: 3'- gGGGGGGUCGUGAaacgaAAG-UGGcu -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 35908 | 0.7 | 0.822658 |
Target: 5'- gUCCCCCCAGUACUccgGCgagagaccuCCGAc -3' miRNA: 3'- -GGGGGGGUCGUGAaa-CGaaagu----GGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 154934 | 0.69 | 0.853914 |
Target: 5'- gUCCCCCCAGUACUccgGCgaggagaccuCCGAc -3' miRNA: 3'- -GGGGGGGUCGUGAaa-CGaaagu-----GGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 10728 | 0.69 | 0.86622 |
Target: 5'- gUCCCCCGGCGCg--Ga---CACCGAc -3' miRNA: 3'- gGGGGGGUCGUGaaaCgaaaGUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 27318 | 0.69 | 0.86622 |
Target: 5'- gUCCCCgAGCACUaUUGUcccgUCGCCGu -3' miRNA: 3'- gGGGGGgUCGUGA-AACGaa--AGUGGCu -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 149716 | 0.69 | 0.86622 |
Target: 5'- aUCCCCUagaAGCGC-UUGCUguaguggCGCCGGc -3' miRNA: 3'- -GGGGGGg--UCGUGaAACGAaa-----GUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 81147 | 0.68 | 0.887809 |
Target: 5'- aCCCCCacaGGCACgc-GCUgaUCACCa- -3' miRNA: 3'- gGGGGGg--UCGUGaaaCGAa-AGUGGcu -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 104937 | 0.68 | 0.894557 |
Target: 5'- -aCCCCCAGCcg--UGCcaagCGCCGAg -3' miRNA: 3'- ggGGGGGUCGugaaACGaaa-GUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 160339 | 0.68 | 0.901072 |
Target: 5'- aCCUCCCCGGCAUgugUGCUagaaagaUACCa- -3' miRNA: 3'- -GGGGGGGUCGUGaa-ACGAaa-----GUGGcu -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 61447 | 0.68 | 0.907353 |
Target: 5'- gCUCCUCGGCGCcgagUUGCUcgagCGCCGu -3' miRNA: 3'- gGGGGGGUCGUGa---AACGAaa--GUGGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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