Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24346 | 5' | -54.5 | NC_005264.1 | + | 82704 | 0.66 | 0.960662 |
Target: 5'- gCCCCCCCaauAGCGCauacUUGCUcugCGCUu- -3' miRNA: 3'- -GGGGGGG---UCGUGa---AACGAaa-GUGGcu -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 38376 | 0.66 | 0.960662 |
Target: 5'- cCUCCUCCGGCGagagcgGCga-CGCCGAg -3' miRNA: 3'- -GGGGGGGUCGUgaaa--CGaaaGUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 157403 | 0.66 | 0.960662 |
Target: 5'- cCUCCUCCGGCGagagcgGCga-CGCCGAg -3' miRNA: 3'- -GGGGGGGUCGUgaaa--CGaaaGUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 29189 | 0.66 | 0.948965 |
Target: 5'- gCUCCCCAgguggcguGCAagugUUGCgugcgUCACCGAu -3' miRNA: 3'- gGGGGGGU--------CGUga--AACGaa---AGUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 85606 | 0.66 | 0.948965 |
Target: 5'- aCCUuuaCCCCAGCGuCUUUaggaaGCg--CGCCGAg -3' miRNA: 3'- -GGG---GGGGUCGU-GAAA-----CGaaaGUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 135802 | 0.66 | 0.948965 |
Target: 5'- aCCCCCCgugcaAGCugUUUGauuaCAUCGAc -3' miRNA: 3'- gGGGGGG-----UCGugAAACgaaaGUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 70317 | 0.66 | 0.948965 |
Target: 5'- uCCCCgCCGGCGC---GCUg--ACCGAg -3' miRNA: 3'- -GGGGgGGUCGUGaaaCGAaagUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 80403 | 0.66 | 0.944602 |
Target: 5'- gCCCCCCCaAGCgACgaaaugGCgcUUAUCGAa -3' miRNA: 3'- -GGGGGGG-UCG-UGaaa---CGaaAGUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 161166 | 0.66 | 0.944602 |
Target: 5'- gCCgCCCCAaUACUUUGCcc-CACCGu -3' miRNA: 3'- -GGgGGGGUcGUGAAACGaaaGUGGCu -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 84864 | 0.66 | 0.940002 |
Target: 5'- gCCCgCUCCGGUACgc-GCgg-CGCCGGc -3' miRNA: 3'- -GGG-GGGGUCGUGaaaCGaaaGUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 55137 | 0.66 | 0.940002 |
Target: 5'- aCCCCgCCguGCGCUgUGCUggCGCa-- -3' miRNA: 3'- -GGGG-GGguCGUGAaACGAaaGUGgcu -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 63425 | 0.66 | 0.938576 |
Target: 5'- aCCgCCCgauGGCGCUUUGCgugccagaggugaaCGCCGAg -3' miRNA: 3'- gGGgGGG---UCGUGAAACGaaa-----------GUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 120990 | 0.67 | 0.933664 |
Target: 5'- cCCCCCCCGagcucccauauGCACUUccuuucccaucuuaUGCUcgccUUCcCCGGu -3' miRNA: 3'- -GGGGGGGU-----------CGUGAA--------------ACGA----AAGuGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 159395 | 0.67 | 0.930083 |
Target: 5'- aCCCUCgUGGCGCUUUGCgagcagCGCgGGc -3' miRNA: 3'- -GGGGGgGUCGUGAAACGaaa---GUGgCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 106302 | 0.67 | 0.930083 |
Target: 5'- uCCCCgCCGGCACcaucaccugUGCcgccggcgCGCCGAu -3' miRNA: 3'- -GGGGgGGUCGUGaa-------ACGaaa-----GUGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 40368 | 0.67 | 0.930083 |
Target: 5'- aCCCUCgUGGCGCUUUGCgagcagCGCgGGc -3' miRNA: 3'- -GGGGGgGUCGUGAAACGaaa---GUGgCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 1964 | 0.67 | 0.929562 |
Target: 5'- cCCCCCCCGagcucccauauGCACUUccuuuccaucuuaUGCUcgccUUCcCCGGu -3' miRNA: 3'- -GGGGGGGU-----------CGUGAA-------------ACGA----AAGuGGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 22062 | 0.67 | 0.924762 |
Target: 5'- uCCCCUCCguGGUACg--GCUUUCGgUGGu -3' miRNA: 3'- -GGGGGGG--UCGUGaaaCGAAAGUgGCU- -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 72138 | 0.67 | 0.913396 |
Target: 5'- aCUCCUCCGGCAUgaUGCUggCGCUc- -3' miRNA: 3'- -GGGGGGGUCGUGaaACGAaaGUGGcu -5' |
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24346 | 5' | -54.5 | NC_005264.1 | + | 61447 | 0.68 | 0.907353 |
Target: 5'- gCUCCUCGGCGCcgagUUGCUcgagCGCCGu -3' miRNA: 3'- gGGGGGGUCGUGa---AACGAaa--GUGGCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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