Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 24347 | 3' | -51.6 | NC_005264.1 | + | 53256 | 0.67 | 0.975026 |
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Target: 5'- cCGCGGGCGCgugucuGGcuuGCUGUCCcgcGGCg -3' miRNA: 3'- cGCGUUCGCGau----UCu--CGAUAGGa--CUG- -5' |
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| 24347 | 3' | -51.6 | NC_005264.1 | + | 90158 | 0.67 | 0.975026 |
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Target: 5'- aCGCAGGCGaucaggGGGGGCgacUCUGACu -3' miRNA: 3'- cGCGUUCGCga----UUCUCGauaGGACUG- -5' |
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| 24347 | 3' | -51.6 | NC_005264.1 | + | 121537 | 0.67 | 0.972255 |
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Target: 5'- cGCGCGacuucAGCGCgGGGGGCgacuUCCcgaGACg -3' miRNA: 3'- -CGCGU-----UCGCGaUUCUCGau--AGGa--CUG- -5' |
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| 24347 | 3' | -51.6 | NC_005264.1 | + | 3819 | 0.67 | 0.969269 |
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Target: 5'- gGUGCucccuGCGCgcgGAGAcCUGcUCCUGGCa -3' miRNA: 3'- -CGCGuu---CGCGa--UUCUcGAU-AGGACUG- -5' |
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| 24347 | 3' | -51.6 | NC_005264.1 | + | 64487 | 0.68 | 0.95895 |
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Target: 5'- -gGCAAGCGCgccgaGAGAGUcg-CCUGGa -3' miRNA: 3'- cgCGUUCGCGa----UUCUCGauaGGACUg -5' |
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| 24347 | 3' | -51.6 | NC_005264.1 | + | 50582 | 0.68 | 0.95895 |
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Target: 5'- aGCGCAcgcGGCGCUGAGcAGCccggcgCUgGACg -3' miRNA: 3'- -CGCGU---UCGCGAUUC-UCGaua---GGaCUG- -5' |
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| 24347 | 3' | -51.6 | NC_005264.1 | + | 99855 | 0.68 | 0.955036 |
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Target: 5'- aGCGCuacugacguGCGCcAAGGGCUgggcgaGUCCgGACg -3' miRNA: 3'- -CGCGuu-------CGCGaUUCUCGA------UAGGaCUG- -5' |
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| 24347 | 3' | -51.6 | NC_005264.1 | + | 97723 | 0.82 | 0.320449 |
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Target: 5'- aGCGCcgccAGGCGCcAAGAGCUGUCC-GACa -3' miRNA: 3'- -CGCG----UUCGCGaUUCUCGAUAGGaCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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