Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24347 | 5' | -52.3 | NC_005264.1 | + | 153340 | 0.69 | 0.94191 |
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Target: 5'- gGCGCCGcgccUGACGC-UGgcUGACUCGc- -3' miRNA: 3'- -CGCGGCuu--ACUGCGuAC--ACUGAGCag -5' |
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| 24347 | 5' | -52.3 | NC_005264.1 | + | 45814 | 0.69 | 0.932069 |
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Target: 5'- cGCGCUGAuc-GCGC-UGU-ACUCGUCa -3' miRNA: 3'- -CGCGGCUuacUGCGuACAcUGAGCAG- -5' |
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| 24347 | 5' | -52.3 | NC_005264.1 | + | 3464 | 0.69 | 0.920674 |
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Target: 5'- gGCGCCcGGUGcCGCAuccacgaUGUGGCacgCGUCg -3' miRNA: 3'- -CGCGGcUUACuGCGU-------ACACUGa--GCAG- -5' |
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| 24347 | 5' | -52.3 | NC_005264.1 | + | 75111 | 0.7 | 0.903154 |
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Target: 5'- gGCGCCGAGcuggGGCGCAgucaaaUGGCcgcgCGUCa -3' miRNA: 3'- -CGCGGCUUa---CUGCGUac----ACUGa---GCAG- -5' |
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| 24347 | 5' | -52.3 | NC_005264.1 | + | 110385 | 0.71 | 0.881473 |
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Target: 5'- cCGCCGAcggcgGGCGCAUGcUGACgcugcucaaaagCGUCg -3' miRNA: 3'- cGCGGCUua---CUGCGUAC-ACUGa-----------GCAG- -5' |
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| 24347 | 5' | -52.3 | NC_005264.1 | + | 146348 | 1.13 | 0.003903 |
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Target: 5'- cGCGCCGAAUGACGCAUGUGACUCGUCg -3' miRNA: 3'- -CGCGGCUUACUGCGUACACUGAGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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