Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 24347 | 5' | -52.3 | NC_005264.1 | + | 69960 | 0.66 | 0.985422 |
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Target: 5'- uGCGUCGGAaGACGCuaccuUGgcGGCUgCGUCc -3' miRNA: 3'- -CGCGGCUUaCUGCGu----ACa-CUGA-GCAG- -5' |
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| 24347 | 5' | -52.3 | NC_005264.1 | + | 26064 | 0.66 | 0.985422 |
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Target: 5'- cGCGCCGAAagGACuCAcUG-GGCUgGUCu -3' miRNA: 3'- -CGCGGCUUa-CUGcGU-ACaCUGAgCAG- -5' |
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| 24347 | 5' | -52.3 | NC_005264.1 | + | 17143 | 0.66 | 0.985422 |
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Target: 5'- gGCGUCGAggGGCGCGgcuacagGauaauccgagaGCUCGUCg -3' miRNA: 3'- -CGCGGCUuaCUGCGUaca----C-----------UGAGCAG- -5' |
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| 24347 | 5' | -52.3 | NC_005264.1 | + | 8413 | 0.66 | 0.987087 |
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Target: 5'- cCGCCGuuuccGCGCAUGUGcCUuUGUCg -3' miRNA: 3'- cGCGGCuuac-UGCGUACACuGA-GCAG- -5' |
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| 24347 | 5' | -52.3 | NC_005264.1 | + | 59709 | 0.66 | 0.987087 |
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Target: 5'- gGCGUCGGuuaucuccGGCGCGcaUGGCUCGUUa -3' miRNA: 3'- -CGCGGCUua------CUGCGUacACUGAGCAG- -5' |
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| 24347 | 5' | -52.3 | NC_005264.1 | + | 25543 | 0.66 | 0.988602 |
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Target: 5'- gGCGCgGAcaaGGCGCuUGUcgcGCUCGUCc -3' miRNA: 3'- -CGCGgCUua-CUGCGuACAc--UGAGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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