Results 61 - 63 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24348 | 5' | -61.4 | NC_005264.1 | + | 135382 | 0.67 | 0.62358 |
Target: 5'- aUGUccuuuGCUGCGCCgCUCGGCGCa--- -3' miRNA: 3'- gACAu----CGGCGCGGgGAGCUGCGgcau -5' |
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24348 | 5' | -61.4 | NC_005264.1 | + | 37338 | 0.67 | 0.62358 |
Target: 5'- --aUGGCCGCGCaCCC-CGAcguuCGCCGc- -3' miRNA: 3'- gacAUCGGCGCG-GGGaGCU----GCGGCau -5' |
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24348 | 5' | -61.4 | NC_005264.1 | + | 54329 | 0.66 | 0.702079 |
Target: 5'- -gGUGGUgGCGCa---CGACGCCGUAc -3' miRNA: 3'- gaCAUCGgCGCGgggaGCUGCGGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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