Results 61 - 64 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24394 | 3' | -65.9 | NC_005264.1 | + | 3428 | 0.73 | 0.185581 |
Target: 5'- --gCgGCGCGCCCCacuuCuCAGGGGUCCg -3' miRNA: 3'- ugaGgCGCGCGGGGc---GcGUCCCCAGG- -5' |
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24394 | 3' | -65.9 | NC_005264.1 | + | 156883 | 0.76 | 0.12098 |
Target: 5'- -aUCCGCGaaugcgccgcUGCCCUGCGCgAGGGGuUCCg -3' miRNA: 3'- ugAGGCGC----------GCGGGGCGCG-UCCCC-AGG- -5' |
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24394 | 3' | -65.9 | NC_005264.1 | + | 37857 | 0.76 | 0.12098 |
Target: 5'- -aUCCGCGaaugcgccgcUGCCCUGCGCgAGGGGuUCCg -3' miRNA: 3'- ugAGGCGC----------GCGGGGCGCG-UCCCC-AGG- -5' |
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24394 | 3' | -65.9 | NC_005264.1 | + | 117075 | 1.09 | 0.000428 |
Target: 5'- cACUCCGCGCGCCCCGCGCAGGGGUCCu -3' miRNA: 3'- -UGAGGCGCGCGGGGCGCGUCCCCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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