Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
24394 | 5' | -54 | NC_005264.1 | + | 126101 | 0.67 | 0.959184 |
Target: 5'- cCGGcGCCGUCGcgcUGCGGGGa----- -3' miRNA: 3'- aGCCuCGGCAGCu--ACGCCUCauaugc -5' |
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24394 | 5' | -54 | NC_005264.1 | + | 136649 | 0.66 | 0.962698 |
Target: 5'- gCGGAGCC-UCGAcgcgcGUGGAGUAa--- -3' miRNA: 3'- aGCCUCGGcAGCUa----CGCCUCAUaugc -5' |
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24394 | 5' | -54 | NC_005264.1 | + | 149430 | 0.68 | 0.933324 |
Target: 5'- gCGGucGCgCGcCGGUGCGGAGagcUGCGg -3' miRNA: 3'- aGCCu-CG-GCaGCUACGCCUCau-AUGC- -5' |
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24394 | 5' | -54 | NC_005264.1 | + | 149591 | 0.7 | 0.848563 |
Target: 5'- -gGGGGCUGcUCGAUGCcGAGUcuuUGCGg -3' miRNA: 3'- agCCUCGGC-AGCUACGcCUCAu--AUGC- -5' |
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24394 | 5' | -54 | NC_005264.1 | + | 150552 | 0.68 | 0.911373 |
Target: 5'- cUCGGgcggggagGGCCGUUGggGUGGGGgcggACGg -3' miRNA: 3'- -AGCC--------UCGGCAGCuaCGCCUCaua-UGC- -5' |
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24394 | 5' | -54 | NC_005264.1 | + | 162805 | 0.68 | 0.911373 |
Target: 5'- aCGGAgacucGCCGUgGGUGCGGGGc--GCa -3' miRNA: 3'- aGCCU-----CGGCAgCUACGCCUCauaUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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